modified_802_ph_10_0

CultureMech:008297 bacterial COMPLEX SOLID_AGAR
Original name: Modified 802 (pH 10.0)

Overview

External ontology
TOGO:M1733 — Modified 802 (pH 10.0)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1733 Original source: NBRC - NBRC_M942 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=942

Composition

flowchart LR
    CultureMech_008297["modified_802_ph_10_0"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008297 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008297 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008297 --> ing2_Yeast_extract
    ing3_K2HPO4["K2HPO4"]
    CultureMech_008297 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008297 --> ing4_Agar__if_needed_

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
900 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 Yeast extract 2 G_PER_L Role: Nutrient source; Properties: Undefined component
4 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
5 Agar (if needed) 15 G_PER_L Role: Solidifying component

Solutions

Na2CO3 solution

Dose: 100 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

Hipolypepton*

Dose: 10 G_PER_L

Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.252827Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1733
2026-01-28T19:48:31.278813Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:52.108897+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:55.479685Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008297
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.035512+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified 802 (pH 10.0)" to "modified_802_ph_10_0" for consistent matching
2026-05-16T09:04:24.960251+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:36.075685+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:25.615077+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:08.479227+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:47.861402+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 60375fbfa4d7083ab6cb67d67fab1fa0e8935ca838fe61e8a5d39aebabeec5cd