modified_802_ph_10_0

CultureMech:008297 bacterial COMPLEX SOLID_AGAR
Original name: Modified 802 (pH 10.0)

Overview

External ontology
TOGO:M1733 — Modified 802 (pH 10.0)
Applications
  • Microbial cultivation
pH
10.0
Notes
TOGO M1733 imports NBRC Medium 942 as Modified 802 (pH 10.0). The NBRC formula lists 100 ml of 10% Na2CO3 solution, 900 ml distilled water, 1 g MgSO4 x 7H2O, 2 g yeast extract, 1 g K2HPO4, 15 g agar if needed, 10 g Hipolypepton, and a final pH near 10.0.

Composition

flowchart LR
    CultureMech_008297["modified_802_ph_10_0"]
    ing0_Na2CO3_solution["Na2CO3 solution"]
    CultureMech_008297 --> ing0_Na2CO3_solution
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3_solution -.-> CHEBI_29377
    ing1_Distilled_water["Distilled water"]
    CultureMech_008297 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008297 --> ing2_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_7H2O -.-> CHEBI_31795
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_008297 --> ing3_Yeast_extract
    ing4_K2HPO4["K2HPO4"]
    CultureMech_008297 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008297 --> ing5_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar__if_needed_ -.-> CHEBI_2509
    ing6_Hipolypepton_["Hipolypepton*"]
    CultureMech_008297 --> ing6_Hipolypepton_

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Na2CO3 solution
CO3.2Na 105.988 g/mol
100.0 ML_PER_L CHEBI:29377
sodium carbonate
 TOGO M1733 / NBRC Medium 942 lists 100 ml/L of 10% Na2CO3 solution, added aseptically after autoclaving.
2 Distilled water
H2O 18.015 g/mol
900.0 ML_PER_L CHEBI:15377
water
 TOGO M1733 / NBRC Medium 942 lists 900 ml/L Distilled water.
3 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
1.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 TOGO M1733 / NBRC Medium 942 lists 1 g/L MgSO4 x 7H2O.
4 Yeast extract 2.0 G_PER_L FOODON:03315426
Yeast extract
 TOGO M1733 / NBRC Medium 942 lists 2 g/L Yeast extract.
5 K2HPO4
HO4P.2K 174.174 g/mol
1.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 TOGO M1733 / NBRC Medium 942 lists 1 g/L K2HPO4.
6 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M1733 / NBRC Medium 942 lists 15 g/L Agar if needed.
7 Hipolypepton* 10.0 G_PER_L FOODON:03315306
Polypeptone
 TOGO M1733 / NBRC Medium 942 lists 10 g/L Hipolypepton with an asterisked Wako Pure Chemical Industries note.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.252827Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1733
2026-01-28T19:48:31.278813Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:52.108897+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:55.479685Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008297
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.035512+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified 802 (pH 10.0)" to "modified_802_ph_10_0" for consistent matching
2026-05-16T09:04:24.960251+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:36.075685+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:25.615077+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:08.479227+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00 repair_togo_m1733_modified_802_score15.py RESOLVED_TOGO_M1733_SCORE15 TOGO M1733 imports NBRC Medium 942 as Modified 802 (pH 10.0). The NBRC formula lists 100 ml of 10% Na2CO3 solution, 900 ml distilled water, 1 g MgSO4 x 7H2O, 2 g yeast extract, 1 g K2HPO4, 15 g agar if needed, 10 g Hipolypepton, and a final pH near 10.0. Corrected the imported water and Na2CO3 solution units, restored Hipolypepton as an ingredient, removed empty solution stubs, added the source pH, and grounded every ingredient.