Overview
- External ontology
- TOGO:M1760 — Nutrient Broth + 5% NaCl (pH 10.0)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1760 Original source: NBRC - NBRC_M972 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=972
Composition
flowchart LR
CultureMech_008325["nutrient_broth_5_nacl_ph_10_0"]
ing0_Distilled_water["Distilled water"]
CultureMech_008325 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008325 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008325 --> ing2_Agar__if_needed_
ing3_Nutrient_Broth__OXOID_["Nutrient Broth (OXOID)"]
CultureMech_008325 --> ing3_Nutrient_Broth__OXOID_
ing4_NaHCO3["NaHCO3"]
CultureMech_008325 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Na2CO3__anhydrous_["Na2CO3 (anhydrous)"]
CultureMech_008325 --> ing5_Na2CO3__anhydrous_
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 900.0, 100.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
50 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 4 | Nutrient Broth (OXOID) | 13 G_PER_L | Properties: Undefined component, Complex component | |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 | Na2CO3 (anhydrous) | 5.3 G_PER_L | Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
Solutions
Sodium-sesquicarbonate solution*
Dose: 100 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nutrient_broth_5_nacl_ph_10_0 (CultureMech:008325)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.296721Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1760 |
| 2026-01-28T19:48:37.695914Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:02.930049+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:34.804766Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:55.712475Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008325 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.254882+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient Broth + 5% NaCl (pH 10.0)" to "nutrient_broth_5_nacl_ph_10_0" for consistent matching |
| 2026-05-16T09:04:27.926692+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:40.167140+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:51.701119+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1165170b5ffc1b4155a8c1a730f2f8622eb299e7b46739c9d2842c010f1c89a5 |