nutrient_broth_5_nacl_ph_10_0

CultureMech:008325 bacterial COMPLEX SOLID_AGAR
Original name: Nutrient Broth + 5% NaCl (pH 10.0)

Overview

External ontology
TOGO:M1760 — Nutrient Broth + 5% NaCl (pH 10.0)
Applications
  • Microbial cultivation
pH
10.0
Notes
TOGO M1760 imports NBRC Medium 972: a 900 ml main base with 13 g Nutrient Broth (OXOID), 50 g NaCl, 15 g agar if needed, and a 100 ml sodium-sesquicarbonate stock containing 4.2 g NaHCO3 and 5.3 g anhydrous Na2CO3; the final pH is 10.0.

Composition

flowchart LR
    CultureMech_008325["nutrient_broth_5_nacl_ph_10_0"]
    ing0_Nutrient_Broth__OXOID_["Nutrient Broth (OXOID)"]
    CultureMech_008325 --> ing0_Nutrient_Broth__OXOID_
    ing1_NaCl["NaCl"]
    CultureMech_008325 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_NaHCO3["NaHCO3"]
    CultureMech_008325 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_Na2CO3["Na2CO3"]
    CultureMech_008325 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008325 --> ing4_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar__if_needed_ -.-> CHEBI_2509
    ing5_Distilled_water["Distilled water"]
    CultureMech_008325 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Nutrient Broth (OXOID) 13.0 G_PER_L TOGO M1760 and NBRC Medium 972 list 13.0 g/L Nutrient Broth (OXOID); retained as an intentionally opaque commercial product.
2 NaCl
Cl.Na 58.443 g/mol
50.0 G_PER_L CHEBI:26710
sodium chloride
 TOGO M1760 and NBRC Medium 972 list 50.0 g/L NaCl.
3 NaHCO3
CHO3.Na 84.006 g/mol
4.2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 NBRC Medium 972 lists 4.2 g NaHCO3 in the separately sterilized 100 ml sodium-sesquicarbonate stock added per liter.
4 Na2CO3
CO3.2Na 105.988 g/mol
5.3 G_PER_L CHEBI:29377
sodium carbonate
 NBRC Medium 972 lists 5.3 g anhydrous Na2CO3 in the separately sterilized 100 ml sodium-sesquicarbonate stock added per liter.
5 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M1760 and NBRC Medium 972 list 15.0 g/L Agar if needed for solid medium.
6 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 NBRC Medium 972 uses 900 ml Distilled water in the main base plus 100 ml water in the sodium-sesquicarbonate stock.

Preparation

  1. Suspend Nutrient Broth (OXOID), NaCl, and optional agar in 900 ml distilled water.
  2. Autoclave the nutrient-broth NaCl base.
  3. Sterilize the 100 ml NaHCO3 and Na2CO3 sodium-sesquicarbonate stock separately.
  4. Aseptically add the sterile sodium-sesquicarbonate stock to the sterile base.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.296721Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1760
2026-01-28T19:48:37.695914Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:02.930049+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:34.804766Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:55.712475Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008325
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.254882+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient Broth + 5% NaCl (pH 10.0)" to "nutrient_broth_5_nacl_ph_10_0" for consistent matching
2026-05-16T09:04:27.926692+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:40.167140+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_nutrient_broths_score15.py RESOLVED_TOGO_M1760_NBRC_972 TOGO M1760 imports NBRC Medium 972: a 900 ml main base with 13 g Nutrient Broth (OXOID), 50 g NaCl, 15 g agar if needed, and a 100 ml sodium-sesquicarbonate stock containing 4.2 g NaHCO3 and 5.3 g anhydrous Na2CO3; the final pH is 10.0.