Overview
- External ontology
- TOGO:M194 — BSK Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M194 Original source: JCM - JCM_M201 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=201 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008529["bsk_medium"]
ing0_gelatin["gelatin"]
CultureMech_008529 --> ing0_gelatin
CHEBI_5291["CHEBI:5291"]
ing0_gelatin -.-> CHEBI_5291
ing1_Distilled_water["Distilled water"]
CultureMech_008529 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_NaHCO3["NaHCO3"]
CultureMech_008529 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008529 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_HEPES["HEPES"]
CultureMech_008529 --> ing4_HEPES
CHEBI_46756["CHEBI:46756"]
ing4_HEPES -.-> CHEBI_46756
ing5_Trisodium_citrate["Trisodium citrate"]
CultureMech_008529 --> ing5_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Trisodium_citrate -.-> CHEBI_53258
ing6_N__Acetyl__D__glucosamine["N--Acetyl--D--glucosamine"]
CultureMech_008529 --> ing6_N__Acetyl__D__glucosamine
ing7_Tryptone["Tryptone"]
CultureMech_008529 --> ing7_Tryptone
ing8_Yeast_extract["Yeast extract"]
CultureMech_008529 --> ing8_Yeast_extract
ing9_Sodium_chloride["Sodium chloride"]
CultureMech_008529 --> ing9_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing9_Sodium_chloride -.-> CHEBI_26710
ing10_Glucose["Glucose"]
CultureMech_008529 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11_CMRL_1066__Gibco_330__1540_["CMRL 1066 (Gibco 330--1540)"]
CultureMech_008529 --> ing11_CMRL_1066__Gibco_330__1540_
ing12_Neopeptone__BD_Difco_["Neopeptone (BD-Difco)"]
CultureMech_008529 --> ing12_Neopeptone__BD_Difco_
ing13_sterile_rabbit_serum["sterile rabbit serum"]
CultureMech_008529 --> ing13_sterile_rabbit_serum
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | gelatin | 200 G_PER_L |
CHEBI:5291
gelatin
|
Role: Solidifying component; Properties: Undefined component |
| 2 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.8 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 | HEPES | 6 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 6 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.7 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Defined component, Organic compound |
| 7 | N--Acetyl--D--glucosamine | 0.4 G_PER_L | Role: Carbon source; Properties: Simple component, Defined component, Organic compound | |
| 8 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 9 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 10 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 12 | CMRL 1066 (Gibco 330--1540) | 100 G_PER_L | Properties: Defined component, Complex component | |
| 13 | Neopeptone (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 14 | sterile rabbit serum | variable VARIABLE | Role: Nutrient source; Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M194_BSK_Medium (CultureMech:008529)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.590428Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M194 |
| 2026-01-28T19:48:11.568652Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: sterile rabbit serum |
| 2026-03-13T06:19:57.788673Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008529 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.412646+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BSK Medium" to "bsk_medium" for consistent matching |
| 2026-05-16T09:03:11.910095+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.049967+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:10.137283+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d6555dbbde26e7e50193e02092b67f4e6839cb74b7c58596d62a5fa87bbbb217 |