bsk_medium

CultureMech:008529 bacterial COMPLEX LIQUID
Original name: BSK Medium

Overview

External ontology
TOGO:M194 — BSK Medium
Applications
  • Microbial cultivation
pH
7.6
Notes
Source: https://togomedium.org/medium/M194 Original source: JCM - JCM_M201 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=201 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The exact recipe specifies albumin, not LB broth. Remove only the three reviewed LB supplier nodes and restore albumin at its source-defined preparation stage. Genuine yeast extract and unrelated annotations are preserved. The original JCM201 endpoint returned no recipe in the pinned capture. This formulation is TOGO-provider-backed, not original-page corroborated.

Composition

flowchart LR
    CultureMech_008529["bsk_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008529 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_008529 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_008529 --> ing2_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing2_Sodium_pyruvate -.-> CHEBI_50144
    ing3_HEPES["HEPES"]
    CultureMech_008529 --> ing3_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing3_HEPES -.-> CHEBI_46756
    ing4_Trisodium_citrate["Trisodium citrate"]
    CultureMech_008529 --> ing4_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing4_Trisodium_citrate -.-> CHEBI_53258
    ing5_N__Acetyl__D__glucosamine["N--Acetyl--D--glucosamine"]
    CultureMech_008529 --> ing5_N__Acetyl__D__glucosamine
    ing6_Bovine_albumin__Fraction_V["Bovine albumin, Fraction V"]
    CultureMech_008529 --> ing6_Bovine_albumin__Fraction_V
    ing7_Glucose["Glucose"]
    CultureMech_008529 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Neopeptone__BD_Difco_["Neopeptone (BD-Difco)"]
    CultureMech_008529 --> ing8_Neopeptone__BD_Difco_

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
0.9 L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component Source water batch input: 900 ml = 0.9 L. This is not a measured final volume.
2 NaHCO3
CHO3.Na 84.006 g/mol
CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component Source batch input: 2.2 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
3 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
CHEBI:50144
sodium pyruvate
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound Source batch input: 0.8 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
4 HEPES CHEBI:46756
HEPES
 Role: Buffer; Properties: Organic compound, Simple component, Defined component Source batch input: 6 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
5 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
CHEBI:53258
sodium citrate
 Role: Buffer; Properties: Simple component, Defined component, Organic compound Source batch input: 0.7 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
6 N--Acetyl--D--glucosamine Role: Carbon source; Properties: Simple component, Defined component, Organic compound Source batch input: 0.4 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
7 Bovine albumin, Fraction V Source batch input: 50 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration.
8 Glucose
C6H12O6 180.156 g/mol
CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component Source batch input: 5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
9 Neopeptone (BD-Difco) Properties: Undefined component Source batch input: 5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.

Preparation

  1. Mix all ingredients in a beaker with a stir bar.
  2. Adjust pH to 7.6.
  3. Add 200 ml of 7% gelatin.
  4. Filter--sterilize entire medium.
  5. Add sterile rabbit serum to final concentration of 6%.

Solutions

7% gelatin solution

Dose: 0.2 L

Stock composition
IngredientConcentrationNotes
gelatin Role: Solidifying component; Properties: Undefined component Source label is 7% gelatin, with unspecified percentage basis and no stock preparation mass. The enclosing descriptor holds the single 200 ml batch dose; the row and comment are the same addition.
Source batch dose: 200 ml = 0.2 L per receiving batch, not g/L or a dose per final liter.

CMRL 1066 (Gibco 330--1540)

Dose: 0.1 L

Properties: Defined component, Complex component Source batch dose: 100 ml = 0.1 L per receiving batch, not g/L or a dose per final liter. CMRL 1066 (Gibco 330--1540) is a product input; its composition and stock strength are not supplied. Do not invent an expanded formulation.

sterile rabbit serum

Role: Nutrient source; Properties: Undefined component Source specifies final 6% sterile rabbit serum without a percentage basis or fixed addition volume. Remove the unsupported VARIABLE placeholder; no 6% v/v strength or fixed dose is asserted. Serum is added after medium filtration.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.590428Z togo-import Imported from TOGO Medium Source: JCM, ID: M194
2026-01-28T19:48:11.568652Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: sterile rabbit serum
2026-03-13T06:19:57.788673Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008529
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.412646+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BSK Medium" to "bsk_medium" for consistent matching
2026-05-16T09:03:11.910095+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:35.049967+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-08T21:45:30.677002+00:00 codex RESTORED_ALBUMIN_COUNTERPART_IDENTITIES_AND_STOCK_CONCENTRATIONS_WITH_EVIDENCE Restore albumin and remove false LB constituents; correct mass/dose and stock/receiving-batch scopes. Retain exact source batch masses without assumed final volume. Unspecified percentage bases remain unknown. No growth claims or new chemical IDs are inferred.