Overview
- External ontology
- TOGO:M1968 — R medium + 5% NaCl
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1968 Original source: NBRC - NBRC_M1246 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1246
Composition
flowchart LR
CultureMech_008549["r_medium_5_nacl"]
ing0_Distilled_water["Distilled water"]
CultureMech_008549 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008549 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008549 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008549 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Tween_80["Tween 80"]
CultureMech_008549 --> ing4_Tween_80
CHEBI_53426["CHEBI:53426"]
ing4_Tween_80 -.-> CHEBI_53426
ing5_Glycerol["Glycerol"]
CultureMech_008549 --> ing5_Glycerol
CHEBI_17754["CHEBI:17754"]
ing5_Glycerol -.-> CHEBI_17754
ing6_Agar__if_needed_["Agar (if needed)"]
CultureMech_008549 --> ing6_Agar__if_needed_
ing7_Malt_extract["Malt extract"]
CultureMech_008549 --> ing7_Malt_extract
ing8_Beef_extract["Beef extract"]
CultureMech_008549 --> ing8_Beef_extract
ing9_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
CultureMech_008549 --> ing9_Bacto_Casamino_Acids__Difco_
ing10_Peptone["Peptone"]
CultureMech_008549 --> ing10_Peptone
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
50 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.05 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Simple component, Organic compound, Defined component |
| 6 |
Glycerol
C3H8O3 92.094 g/mol
|
2 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 8 | Malt extract | 5 G_PER_L | Properties: Undefined component | |
| 9 | Beef extract | 2 G_PER_L | Properties: Undefined component | |
| 10 | Bacto Casamino Acids (Difco) | 5 G_PER_L | Properties: Undefined component | |
| 11 | Peptone | 10 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- r_medium_5_nacl (CultureMech:008549)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.757580Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1968 |
| 2026-01-28T19:48:27.852051Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:57.938538Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008549 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.564845+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R medium + 5% NaCl" to "r_medium_5_nacl" for consistent matching |
| 2026-06-05T07:41:43.796520+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.090461+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:30.833105+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:54.290315+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f0d96e06799058ce0ae8a18f65f2bc70733ffc7d8bb34b0f27f7250a433d334c |