r_medium_5_nacl

CultureMech:008549 bacterial COMPLEX SOLID_AGAR
Original name: R medium + 5% NaCl

Overview

External ontology
TOGO:M1968 — R medium + 5% NaCl
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M1968 imports NBRC Medium 1246 R medium + 5% NaCl, which uses the NBRC R medium formula with 50 g/L NaCl and final pH 7.0.

Composition

flowchart LR
    CultureMech_008549["r_medium_5_nacl"]
    ing0_Peptone["Peptone"]
    CultureMech_008549 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008549 --> ing1_Yeast_extract
    ing2_Malt_extract["Malt extract"]
    CultureMech_008549 --> ing2_Malt_extract
    ing3_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
    CultureMech_008549 --> ing3_Bacto_Casamino_Acids__Difco_
    ing4_Beef_extract["Beef extract"]
    CultureMech_008549 --> ing4_Beef_extract
    ing5_Glycerol["Glycerol"]
    CultureMech_008549 --> ing5_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing5_Glycerol -.-> CHEBI_17754
    ing6_Tween_80["Tween 80"]
    CultureMech_008549 --> ing6_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing6_Tween_80 -.-> CHEBI_53426
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008549 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_NaCl["NaCl"]
    CultureMech_008549 --> ing8_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing8_NaCl -.-> CHEBI_26710
    ing9_Distilled_water["Distilled water"]
    CultureMech_008549 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377
    ing10_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008549 --> ing10_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing10_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Peptone 10.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 NBRC Medium 1246 lists 10.0 g/L Peptone.
2 Yeast extract 5.0 G_PER_L FOODON:03315426
yeast extract
 NBRC Medium 1246 lists 5.0 g/L Yeast extract.
3 Malt extract 5.0 G_PER_L FOODON:03301056
malt extract
 NBRC Medium 1246 lists 5.0 g/L Malt extract.
4 Bacto Casamino Acids (Difco) 5.0 G_PER_L FOODON:03315719
mammalian milk protein (hydrolyzed)
 NBRC Medium 1246 lists 5.0 g/L Bacto Casamino Acids (Difco).
5 Beef extract 2.0 G_PER_L FOODON:03302088
beef extract
 NBRC Medium 1246 lists 2.0 g/L Beef extract.
6 Glycerol
C3H8O3 92.094 g/mol
2.0 G_PER_L CHEBI:17754
glycerol
 NBRC Medium 1246 lists 2.0 g/L Glycerol.
7 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
0.05 G_PER_L CHEBI:53426
polysorbate 80
 NBRC Medium 1246 lists 0.05 g/L Tween 80.
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 NBRC Medium 1246 lists 1.0 g/L MgSO4 x 7 H2O.
9 NaCl
Cl.Na 58.443 g/mol
50.0 G_PER_L CHEBI:26710
sodium chloride
 NBRC Medium 1246 lists 50.0 g/L NaCl.
10 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 NBRC Medium 1246 lists 1.0 L Distilled water.
11 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 NBRC Medium 1246 lists 15.0 g/L Agar if needed.

Preparation

  1. Dissolve peptone, yeast extract, malt extract, Bacto Casamino Acids, beef extract, glycerol, Tween 80, magnesium sulfate heptahydrate, NaCl, and agar if needed in 1.0 L distilled water.
  2. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.757580Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1968
2026-01-28T19:48:27.852051Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:57.938538Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008549
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.564845+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R medium + 5% NaCl" to "r_medium_5_nacl" for consistent matching
2026-06-05T07:41:43.796520+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.090461+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:58:30.833105+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-09-12T00:00:00-07:00 repair_togo_m1482_m1968_r_medium_score15.py RESOLVED_TOGO_M1968_R_MEDIUM_5_NACL_SCORE15 Corrected the imported distilled-water unit from g/L to L, added pH 7.0, grounded all eleven disclosed components, and linked this record to NBRC Medium 264 R medium as a salinity variant.