lb_streptomycin_rifampicin_medium

CultureMech:008554 bacterial COMPLEX SOLID_AGAR
Original name: LB + Streptomycin, Rifampicin medium

Overview

External ontology
TOGO:M1972 — LB + Streptomycin, Rifampicin medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'lb_ampicilin_hygromycin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M1936', 'original_category': 'bacterial'}
  • {'name': 'lb_ampicillin_cefpodoxime_kanamycin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M2012', 'original_category': 'bacterial'}
  • {'name': 'lb_ampicillin_kanamycin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M2050', 'original_category': 'bacterial'}
  • {'name': 'lb_chloramphenicol_medium', 'source': 'TOGO', 'source_id': 'TOGO:M1899', 'original_category': 'bacterial'}
  • {'name': 'lb_kanamycin_hygromycin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M1935', 'original_category': 'bacterial'}
  • {'name': 'lb_nalidixic_acid_ciprofloxacin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M2013', 'original_category': 'bacterial'}
  • {'name': 'lb_rifampicin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M1971', 'original_category': 'bacterial'}
  • {'name': 'lb_streptomycin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M1970', 'original_category': 'bacterial'}
Notes
Source: https://togomedium.org/medium/M1972 Original source: NBRC - NBRC_M1250 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1250

Composition

flowchart LR
    CultureMech_008554["lb_streptomycin_rifampicin_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008554 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008554 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_008554 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008554 --> ing3_Agar__if_needed_
    ing4_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
    CultureMech_008554 --> ing4_Bacto_Tryptone__Difco_

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
4 Agar (if needed) 15 G_PER_L Role: Solidifying component
5 Bacto Tryptone (Difco) 10 G_PER_L Properties: Undefined component

Solutions

Rifampicin solution (50 mg/ml)*

Dose: 5 G_PER_L

Role: Antimicrobial agent; Properties: Defined component, Organic compound, Solution

Streptomycin solution (50 mg/ml)*

Dose: 10 G_PER_L

Role: Antimicrobial agent; Properties: Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 9 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:58.762233Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1972
2026-01-28T19:48:41.093805Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:09.165520+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:57.966423Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008554
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.591403+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB + Streptomycin, Rifampicin medium" to "lb_streptomycin_rifampicin_medium" for consistent matching
2026-05-16T09:03:38.907281+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:10.949540+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:40.015066+00:00 recipe-merger Merged 9 duplicate recipes into canonical record Sources: lb_ampicilin_hygromycin_medium, lb_ampicillin_cefpodoxime_kanamycin_medium, lb_ampicillin_kanamycin_medium, lb_chloramphenicol_medium, lb_kanamycin_hygromycin_medium, lb_nalidixic_acid_ciprofloxacin_medium, lb_rifampicin_medium, lb_streptomycin_medium, lb_streptomycin_rifampicin_medium
2026-08-06T03:05:40.015137+00:00 merge_recipes.py MERGED_RECIPES merged 9 source recipe(s) on fingerprint 99e3b177df198fce534b889fa8b5168357f0b85ef91ad229d32c60485c0c9f7d