lb_ampicillin_kanamycin_medium

CultureMech:008641 bacterial COMPLEX SOLID_AGAR
Original name: LB + Ampicillin, Kanamycin medium

Overview

External ontology
TOGO:M2050 — LB + Ampicillin, Kanamycin medium
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M2050 imports NBRC Medium 1350 LB + Ampicillin, Kanamycin medium. NBRC 1350 lists a high-salt LB base with 10 g/L Bacto Tryptone (Difco), 5 g/L yeast extract, 10 g/L NaCl, 1.0 ml/L Ampicillin solution (50 mg/ml), 1.0 ml/L Kanamycin solution (50 mg/ml), 15 g/L agar if needed, and pH 7.0.

Composition

flowchart LR
    CultureMech_008641["lb_ampicillin_kanamycin_medium"]
    ing0_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
    CultureMech_008641 --> ing0_Bacto_Tryptone__Difco_
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008641 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_008641 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Distilled_water["Distilled water"]
    CultureMech_008641 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008641 --> ing4_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Bacto Tryptone (Difco) 10 G_PER_L NBRC Medium 1350 lists Bacto Tryptone (Difco) as a source-disclosed digest product not reducible to one ChEBI molecule.
2 Yeast extract 5 G_PER_L FOODON:03315426
yeast extract
 TOGO M2050 / NBRC Medium 1350 lists 5 g/L Yeast extract.
3 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
 TOGO M2050 / NBRC Medium 1350 lists 10 g/L NaCl.
4 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 TOGO M2050 / NBRC Medium 1350 lists 1000 ml/L Distilled water.
5 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 TOGO M2050 / NBRC Medium 1350 lists 15 g/L agar if needed.

Preparation

  1. Prepare the high-salt LB base from 10 g/L Bacto Tryptone (Difco), 5 g/L yeast extract, 10 g/L NaCl, 1000 ml/L distilled water, and 15 g/L agar if needed.
  2. Sterilize the 50 mg/ml ampicillin and 50 mg/ml kanamycin stocks separately by filtration.
  3. Add 1.0 ml/L each filter-sterilized Ampicillin solution (50 mg/ml) and Kanamycin solution (50 mg/ml) to the high-salt LB base.

Solutions

Ampicillin solution (50 mg/ml)

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Ampicillin 50.0 MG_PER_ML NBRC Medium 1350 specifies this stock as 50 mg/ml Ampicillin.
TOGO M2050 / NBRC Medium 1350 lists 1 ml/L Ampicillin solution (50 mg/ml).

Kanamycin solution (50 mg/ml)

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Kanamycin 50.0 MG_PER_ML NBRC Medium 1350 specifies this stock as 50 mg/ml Kanamycin.
TOGO M2050 / NBRC Medium 1350 lists 1 ml/L Kanamycin solution (50 mg/ml).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.962971Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2050
2026-01-28T19:48:40.454257Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:08.199535+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:58.971375Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008641
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.592585+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB + Ampicillin, Kanamycin medium" to "lb_ampicillin_kanamycin_medium" for consistent matching
2026-05-16T09:03:38.799993+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:10.834108+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2050_lb_ampicillin_kanamycin_score15.py RESOLVED_TOGO_M2050_LB_AMPICILLIN_KANAMYCIN_SCORE15 TOGO M2050 imports NBRC Medium 1350 LB + Ampicillin, Kanamycin medium. NBRC 1350 lists a high-salt LB base with 10 g/L Bacto Tryptone (Difco), 5 g/L yeast extract, 10 g/L NaCl, 1.0 ml/L Ampicillin solution (50 mg/ml), 1.0 ml/L Kanamycin solution (50 mg/ml), 15 g/L agar if needed, and pH 7.0. Corrected the imported distilled-water row from a g/L artifact to an ml/L volume and nested the separately filter-sterilized ampicillin and kanamycin stocks.