nms_modified_for_gela4

CultureMech:008567 bacterial COMPLEX SOLID_AGAR
Original name: NMS modified for Gela4

Overview

External ontology
TOGO:M1984 — NMS modified for Gela4
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1984 Original source: NBRC - NBRC_M1270 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1270

Composition

flowchart LR
    CultureMech_008567["nms_modified_for_gela4"]
    ing0_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008567 --> ing0_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing0_CaCl2_2H2O -.-> CHEBI_86158
    ing1_NH4Cl["NH4Cl"]
    CultureMech_008567 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Methanol["Methanol"]
    CultureMech_008567 --> ing2_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing2_Methanol -.-> CHEBI_17790
    ing3_MgSO4_6H2O["MgSO4·6H2O"]
    CultureMech_008567 --> ing3_MgSO4_6H2O
    CHEBI_32599["CHEBI:32599"]
    ing3_MgSO4_6H2O -.-> CHEBI_32599
    ing4_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008567 --> ing4_Agar__if_needed_
    ing5_Fe_EDTA["Fe·EDTA"]
    CultureMech_008567 --> ing5_Fe_EDTA
    ing6_Distilled_water["Distilled water"]
    CultureMech_008567 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_KH2PO4["KH2PO4"]
    CultureMech_008567 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_Na2HPO4_12H2O["Na2HPO4·12H2O"]
    CultureMech_008567 --> ing8_Na2HPO4_12H2O
    CHEBI_34683["CHEBI:34683"]
    ing8_Na2HPO4_12H2O -.-> CHEBI_34683
    ing9_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008567 --> ing9_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing9_Na2MoO4_2H2O -.-> CHEBI_75213
    ing10_H3BO3["H3BO3"]
    CultureMech_008567 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_008567 --> ing11_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_7H2O -.-> CHEBI_75836
    ing12_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008567 --> ing12_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_4H2O -.-> CHEBI_86368
    ing13_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008567 --> ing13_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing13_CoCl2_6H2O -.-> CHEBI_35696
    ing14_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_008567 --> ing14_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_6H2O -.-> CHEBI_34887
    ing15_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_008567 --> ing15_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_7H2O -.-> CHEBI_32312
    ing16_CuSO4_5H2O["CuSO4·5H2O"]
    CultureMech_008567 --> ing16_CuSO4_5H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_5H2O -.-> CHEBI_31440
    ing17_EDTA_2Na["EDTA·2Na"]
    CultureMech_008567 --> ing17_EDTA_2Na
    CHEBI_64734["CHEBI:64734"]
    ing17_EDTA_2Na -.-> CHEBI_64734

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
2 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
3 Methanol
CH4O 32.042 g/mol
5 G_PER_L CHEBI:17790
methanol
 Role: Solvating media; Properties: Organic compound, Defined component, Simple component
4 MgSO4·6H2O
Mg.O4S 120.368 g/mol
1 G_PER_L CHEBI:32599
magnesium sulfate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 Agar (if needed) 15 G_PER_L Role: Solidifying component
6 Fe·EDTA 0.038 G_PER_L Properties: Defined component, Simple component, Organic compound
7 Distilled water
H2O 18.015 g/mol
6.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 5.0]
8 KH2PO4
H2O4P.K 136.084 g/mol
26 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9 Na2HPO4·12H2O
HO4P.2Na 141.958 g/mol
71.6 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
10 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
1.3 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 H3BO3
H3BO3 61.833 g/mol
0.075 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
2.5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 CoCl2·6H2O
2Cl.Co 129.839 g/mol
0.25 G_PER_L CHEBI:35696
cobalt dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15 NiCl2·6H2O
Cl2Ni 129.599 g/mol
0.05 G_PER_L CHEBI:34887
nickel dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
2 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 EDTA·2Na
C10H14N2O8.2Na 336.208 g/mol
1.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
 Properties: Defined component, Organic compound, Simple component

Solutions

Seawater***

Dose: 1 G_PER_L

See mediadive_1801_Seawater.yaml for composition

Phosphate solution*

Dose: 10 G_PER_L

See mediadive_4669_Phosphate_solution.yaml for composition

Trace element solution**

Dose: 0.5 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.796792Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1984
2026-01-28T19:48:33.763618Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:55.991424+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:09:27.535809Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:58.141586Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008567
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.768574+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NMS modified for Gela4" to "nms_modified_for_gela4" for consistent matching
2026-05-16T09:04:27.836037+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:40.040382+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.670033+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:51.600048+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint c7727433d5096b2ab33e5ef06b7deb20a38515b9c75bb0a4b17b33aa39ddbb9b