Overview
- External ontology
- TOGO:M1984 — NMS modified for Gela4
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1984 Original source: NBRC - NBRC_M1270 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1270
Composition
flowchart LR
CultureMech_008567["nms_modified_for_gela4"]
ing0_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008567 --> ing0_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing0_CaCl2_2H2O -.-> CHEBI_86158
ing1_NH4Cl["NH4Cl"]
CultureMech_008567 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Methanol["Methanol"]
CultureMech_008567 --> ing2_Methanol
CHEBI_17790["CHEBI:17790"]
ing2_Methanol -.-> CHEBI_17790
ing3_MgSO4_6H2O["MgSO4·6H2O"]
CultureMech_008567 --> ing3_MgSO4_6H2O
CHEBI_32599["CHEBI:32599"]
ing3_MgSO4_6H2O -.-> CHEBI_32599
ing4_Agar__if_needed_["Agar (if needed)"]
CultureMech_008567 --> ing4_Agar__if_needed_
ing5_Fe_EDTA["Fe·EDTA"]
CultureMech_008567 --> ing5_Fe_EDTA
ing6_Distilled_water["Distilled water"]
CultureMech_008567 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_KH2PO4["KH2PO4"]
CultureMech_008567 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_Na2HPO4_12H2O["Na2HPO4·12H2O"]
CultureMech_008567 --> ing8_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing8_Na2HPO4_12H2O -.-> CHEBI_34683
ing9_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008567 --> ing9_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_2H2O -.-> CHEBI_75213
ing10_H3BO3["H3BO3"]
CultureMech_008567 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008567 --> ing11_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_7H2O -.-> CHEBI_75836
ing12_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008567 --> ing12_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_4H2O -.-> CHEBI_86368
ing13_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008567 --> ing13_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing13_CoCl2_6H2O -.-> CHEBI_35696
ing14_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008567 --> ing14_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_6H2O -.-> CHEBI_34887
ing15_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008567 --> ing15_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_7H2O -.-> CHEBI_32312
ing16_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008567 --> ing16_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_5H2O -.-> CHEBI_31440
ing17_EDTA_2Na["EDTA·2Na"]
CultureMech_008567 --> ing17_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing17_EDTA_2Na -.-> CHEBI_64734
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Methanol
CH4O 32.042 g/mol
|
5 G_PER_L |
CHEBI:17790
methanol
|
Role: Solvating media; Properties: Organic compound, Defined component, Simple component |
| 4 |
MgSO4·6H2O
Mg.O4S 120.368 g/mol
|
1 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 6 | Fe·EDTA | 0.038 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 7 |
Distilled water
H2O 18.015 g/mol
|
6.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 5.0] |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
26 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2HPO4·12H2O
HO4P.2Na 141.958 g/mol
|
71.6 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
1.3 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.075 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.25 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.05 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
EDTA·2Na
C10H14N2O8.2Na 336.208 g/mol
|
1.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Seawater***
Dose: 1 G_PER_L
See mediadive_1801_Seawater.yaml for composition
Phosphate solution*
Dose: 10 G_PER_L
See mediadive_4669_Phosphate_solution.yaml for composition
Trace element solution**
Dose: 0.5 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.796792Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1984 |
| 2026-01-28T19:48:33.763618Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:55.991424+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:27.535809Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:58.141586Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008567 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.768574+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NMS modified for Gela4" to "nms_modified_for_gela4" for consistent matching |
| 2026-05-16T09:04:27.836037+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:40.040382+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.670033+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |