Overview
- External ontology
- TOGO:M2003 — LB + 10mM MgSO4, 3mM CaCl2 and 0.1% Casamino acids
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2003 Original source: NBRC - NBRC_M1290 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1290
Composition
flowchart LR
CultureMech_008590["lb_10mm_mgso4_3mm_cacl2_and_0_1_casamino_acids"]
ing0_Distilled_water["Distilled water"]
CultureMech_008590 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008590 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008590 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008590 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008590 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_Agar__if_needed_["Agar (if needed)"]
CultureMech_008590 --> ing5_Agar__if_needed_
ing6_Tryptone["Tryptone"]
CultureMech_008590 --> ing6_Tryptone
ing7_Casamino_acids["Casamino acids"]
CultureMech_008590 --> ing7_Casamino_acids
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.45 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 6 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 7 | Tryptone | 10 G_PER_L | Properties: Undefined component | |
| 8 | Casamino acids | 3 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- lb_10mm_mgso4_3mm_cacl2_and_0_1_casamino_acids (CultureMech:008590)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.855570Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2003 |
| 2026-01-28T19:48:37.703669Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:58.450714Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008590 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.068594+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB + 10mM MgSO4, 3mM CaCl2 and 0.1% Casamino acids" to "lb_10mm_mgso4_3mm_cacl2_and_0_1_casamino_acids" for consistent matching |
| 2026-06-05T07:41:10.781879+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.320310+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:50.339211+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:39.934337+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f9af2596e3c7334b4c2cbdc5560d2321b61154e8e0905620ee0e852ed1b27b2c |