lb_10mm_mgso4_3mm_cacl2_and_0_1_casamino_acids

CultureMech:008590 bacterial COMPLEX SOLID_AGAR
Original name: LB + 10mM MgSO4, 3mM CaCl2 and 0.1% Casamino acids

Overview

External ontology
TOGO:M2003 — LB + 10mM MgSO4, 3mM CaCl2 and 0.1% Casamino acids
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2003 Original source: NBRC - NBRC_M1290 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1290

Composition

flowchart LR
    CultureMech_008590["lb_10mm_mgso4_3mm_cacl2_and_0_1_casamino_acids"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008590 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008590 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008590 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_008590 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008590 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008590 --> ing5_Agar__if_needed_
    ing6_Tryptone["Tryptone"]
    CultureMech_008590 --> ing6_Tryptone
    ing7_Casamino_acids["Casamino acids"]
    CultureMech_008590 --> ing7_Casamino_acids

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
2.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
3 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
5 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.45 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 Agar (if needed) 15 G_PER_L Role: Solidifying component
7 Tryptone 10 G_PER_L Properties: Undefined component
8 Casamino acids 3 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.855570Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2003
2026-01-28T19:48:37.703669Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:58.450714Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008590
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.068594+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB + 10mM MgSO4, 3mM CaCl2 and 0.1% Casamino acids" to "lb_10mm_mgso4_3mm_cacl2_and_0_1_casamino_acids" for consistent matching
2026-06-05T07:41:10.781879+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:40.320310+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:57:50.339211+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.