Overview
- External ontology
- TOGO:M2036 — Basal Salt Medium with biphenyl
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2036 Original source: NBRC - NBRC_M1333 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1333
Composition
flowchart LR
CultureMech_008625["basal_salt_medium_with_biphenyl"]
ing0_Distilled_water["Distilled water"]
CultureMech_008625 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_biphenyl["biphenyl"]
CultureMech_008625 --> ing1_biphenyl
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008625 --> ing2_Agar__if_needed_
ing3_KH2PO4["KH2PO4"]
CultureMech_008625 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_008625 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KOH["KOH"]
CultureMech_008625 --> ing5_KOH
CHEBI_32035["CHEBI:32035"]
ing5_KOH -.-> CHEBI_32035
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_008625 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_NaOH["NaOH"]
CultureMech_008625 --> ing7_NaOH
CHEBI_32145["CHEBI:32145"]
ing7_NaOH -.-> CHEBI_32145
ing8_FeSO4["FeSO4"]
CultureMech_008625 --> ing8_FeSO4
CHEBI_75832["CHEBI:75832"]
ing8_FeSO4 -.-> CHEBI_75832
ing9_MgCl2["MgCl2"]
CultureMech_008625 --> ing9_MgCl2
CHEBI_6636["CHEBI:6636"]
ing9_MgCl2 -.-> CHEBI_6636
ing10_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008625 --> ing10_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_4H2O -.-> CHEBI_86368
ing11_CaCl2["CaCl2"]
CultureMech_008625 --> ing11_CaCl2
CHEBI_3312["CHEBI:3312"]
ing11_CaCl2 -.-> CHEBI_3312
ing12_Na2MoO4_4H2O["Na2MoO4·4H2O"]
CultureMech_008625 --> ing12_Na2MoO4_4H2O
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1338.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 936.0, 1.0, 1.0, 100.0, 100.0, 100.0, 100.0] |
| 2 | biphenyl | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 3 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
68.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
87.09 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
198.66 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 9 |
FeSO4
Fe.O4S 151.908 g/mol
|
0.056 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MgCl2
2Cl.Mg 95.211 g/mol
|
16.26 G_PER_L |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CaCl2
Ca.2Cl 110.984 g/mol
|
2.65 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 | Na2MoO4·4H2O | 0.1 G_PER_L | Role: Mineral source |
Solutions
20 x PO4*
Dose: 50 G_PER_L
100 x (NH4)2SO4**
Dose: 10 G_PER_L
1000 x FeSO4***
Dose: 1 G_PER_L
1000 x MgCl2****
Dose: 1 G_PER_L
1000 x Trace elements*****
Dose: 1 G_PER_L
1000 x Na2MoO4******
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- basal_salt_medium_with_biphenyl (CultureMech:008625)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.931576Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2036 |
| 2026-01-28T19:48:33.984713Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:56.386719+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:27.943258Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:19:58.823277Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008625 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.443738+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Basal Salt Medium with biphenyl" to "basal_salt_medium_with_biphenyl" for consistent matching |
| 2026-05-16T09:03:25.467043+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:50.543518+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.807937+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.770024+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4b93845f2d2c7159b89d262db19241ceb98b56ee764a409f963f4d68fa766d68 |