basal_salt_medium_with_biphenyl

CultureMech:008625 bacterial COMPLEX SOLID_AGAR
Original name: Basal Salt Medium with biphenyl

Overview

External ontology
TOGO:M2036 — Basal Salt Medium with biphenyl
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2036 Original source: NBRC - NBRC_M1333 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1333

Composition

flowchart LR
    CultureMech_008625["basal_salt_medium_with_biphenyl"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008625 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_biphenyl["biphenyl"]
    CultureMech_008625 --> ing1_biphenyl
    CHEBI_17097["CHEBI:17097"]
    ing1_biphenyl -.-> CHEBI_17097
    ing2_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008625 --> ing2_Agar__if_needed_
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008625 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_008625 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_KOH["KOH"]
    CultureMech_008625 --> ing5_KOH
    CHEBI_32035["CHEBI:32035"]
    ing5_KOH -.-> CHEBI_32035
    ing6__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008625 --> ing6__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing6__NH4_2SO4 -.-> CHEBI_62946
    ing7_NaOH["NaOH"]
    CultureMech_008625 --> ing7_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing7_NaOH -.-> CHEBI_32145
    ing8_FeSO4["FeSO4"]
    CultureMech_008625 --> ing8_FeSO4
    CHEBI_75832["CHEBI:75832"]
    ing8_FeSO4 -.-> CHEBI_75832
    ing9_MgCl2["MgCl2"]
    CultureMech_008625 --> ing9_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing9_MgCl2 -.-> CHEBI_6636
    ing10_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008625 --> ing10_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_4H2O -.-> CHEBI_86368
    ing11_CaCl2["CaCl2"]
    CultureMech_008625 --> ing11_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing11_CaCl2 -.-> CHEBI_3312
    ing12_Na2MoO4_4H2O["Na2MoO4·4H2O"]
    CultureMech_008625 --> ing12_Na2MoO4_4H2O

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1338.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 936.0, 1.0, 1.0, 100.0, 100.0, 100.0, 100.0]
2 biphenyl
C12H10 154.212 g/mol
1 G_PER_L CHEBI:17097
biphenyl
 Role: Carbon source; Properties: Organic compound, Defined component, Simple component
3 Agar (if needed) 15 G_PER_L Role: Solidifying component
4 KH2PO4
H2O4P.K 136.084 g/mol
68.05 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 K2HPO4
HO4P.2K 174.174 g/mol
87.09 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6 KOH
HO.K 56.105 g/mol
variable VARIABLE CHEBI:32035
potassium hydroxide
 Role: Mineral source; Properties: Defined component, Solution
7 (NH4)2SO4
2H4N.O4S 132.141 g/mol
198.66 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
8 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 Properties: Defined component, Inorganic compound, Solution
9 FeSO4
Fe.O4S 151.908 g/mol
0.056 G_PER_L CHEBI:75832
iron(2+) sulfate (anhydrous)
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 MgCl2
2Cl.Mg 95.211 g/mol
16.26 G_PER_L CHEBI:6636
magnesium dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 CaCl2
Ca.2Cl 110.984 g/mol
2.65 G_PER_L CHEBI:3312
calcium dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 Na2MoO4·4H2O 0.1 G_PER_L Role: Mineral source

Solutions

20 x PO4*

Dose: 50 G_PER_L

100 x (NH4)2SO4**

Dose: 10 G_PER_L

1000 x FeSO4***

Dose: 1 G_PER_L

1000 x MgCl2****

Dose: 1 G_PER_L

1000 x Trace elements*****

Dose: 1 G_PER_L

1000 x Na2MoO4******

Dose: 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.931576Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2036
2026-01-28T19:48:33.984713Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: KOH; Added default concentration for ingredient: NaOH
2026-03-09T08:04:56.386719+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T03:09:27.943258Z data-quality-cleanup-v1.0 Data quality fixes Merged 6 duplicate ingredient(s)
2026-03-13T06:19:58.823277Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008625
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.443738+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Basal Salt Medium with biphenyl" to "basal_salt_medium_with_biphenyl" for consistent matching
2026-05-16T09:03:25.467043+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:50.543518+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.807937+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-20T19:01:33.764138+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).