Overview
- External ontology
- TOGO:M2040 — NSSM medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2040 Original source: NBRC - NBRC_M1338 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1338
Composition
flowchart LR
CultureMech_008630["nssm_medium"]
ing0_NaCl["NaCl"]
CultureMech_008630 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_008630 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008630 --> ing2_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Sodium_pyruvate -.-> CHEBI_50144
ing3_Artificial_seawater["Artificial seawater"]
CultureMech_008630 --> ing3_Artificial_seawater
ing4_Glucose["Glucose"]
CultureMech_008630 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Agar__if_needed_["Agar (if needed)"]
CultureMech_008630 --> ing5_Agar__if_needed_
ing6_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008630 --> ing6_Bacto_Yeast_Extract__Difco_
ing7_Casamino_acids["Casamino acids"]
CultureMech_008630 --> ing7_Casamino_acids
ing8_Bacto_Proteose_Peptone_No__3__Difco_["Bacto Proteose Peptone No. 3 (Difco)"]
CultureMech_008630 --> ing8_Bacto_Proteose_Peptone_No__3__Difco_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
132 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.05 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 4 | Artificial seawater | 1 G_PER_L | Role: Mineral source, Solvating media; Properties: Complex component, Defined component, Solution | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 6 | Agar (if needed) | 20 G_PER_L | Role: Solidifying component | |
| 7 | Bacto Yeast Extract (Difco) | 1 G_PER_L | Properties: Undefined component | |
| 8 | Casamino acids | 1 G_PER_L | Properties: Undefined component | |
| 9 | Bacto Proteose Peptone No. 3 (Difco) | 3 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nssm_medium (CultureMech:008630)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.939490Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2040 |
| 2026-01-28T19:48:14.165019Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:58.862536Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008630 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.485403+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NSSM medium" to "nssm_medium" for consistent matching |
| 2026-06-05T07:41:40.092991+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.431504+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:09.693637+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:51.644795+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint be337ef743c4d11402a21ba8cb4095b1e76b3e3cb9467bde74a714db41524781 |