nssm_medium (CultureMech:008630)

Overview

External ontology

TOGO:M2040 — NSSM medium

Applications

Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

TOGO M2040 imports NBRC Medium 1338 NSSM medium. The NBRC source lists 132 g NaCl, 0.6 g K2HPO4, 0.05 g sodium pyruvate, 1 L artificial seawater, 1 g glucose, 20 g agar, 1 g Bacto Yeast Extract, 1 g Casamino acids, and 3 g Bacto Proteose Peptone No. 3; pH is 7.0-7.2.

Composition

flowchart LR
    CultureMech_008630["nssm_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_008630 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_008630 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_008630 --> ing2_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing2_Sodium_pyruvate -.-> CHEBI_50144
    ing3_Artificial_seawater["Artificial seawater"]
    CultureMech_008630 --> ing3_Artificial_seawater
    ing4_Glucose["Glucose"]
    CultureMech_008630 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Agar["Agar"]
    CultureMech_008630 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_008630 --> ing6_Bacto_Yeast_Extract__Difco_
    ing7_Casamino_acids["Casamino acids"]
    CultureMech_008630 --> ing7_Casamino_acids
    ing8_Bacto_Proteose_Peptone_No__3__Difco_["Bacto Proteose Peptone No. 3 (Difco)"]
    CultureMech_008630 --> ing8_Bacto_Proteose_Peptone_No__3__Difco_

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	NaCl Cl.Na 58.443 g/mol	132.0 G_PER_L	CHEBI:26710 sodium chloride	TOGO M2040 and NBRC Medium 1338 list 132 g/L NaCl.
2	K2HPO4 HO4P.2K 174.174 g/mol	0.6 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	TOGO M2040 and NBRC Medium 1338 list 0.6 g/L K2HPO4.
3	Sodium pyruvate C3H3O3.Na 110.044 g/mol	0.05 G_PER_L	CHEBI:50144 sodium pyruvate	TOGO M2040 and NBRC Medium 1338 list 0.05 g/L Sodium pyruvate.
4	Artificial seawater	1.0 L		TOGO M2040 and NBRC Medium 1338 list 1 L Artificial seawater as the solvent matrix.
5	Glucose C6H12O6 180.156 g/mol	1.0 G_PER_L	CHEBI:17234 glucose	TOGO M2040 and NBRC Medium 1338 list 1.0 g/L Glucose.
6	Agar	20.0 G_PER_L	CHEBI:2509 agar	TOGO M2040 and NBRC Medium 1338 list 20.0 g/L Agar.
7	Bacto Yeast Extract (Difco)	1.0 G_PER_L		TOGO M2040 and NBRC Medium 1338 list 1.0 g/L Bacto Yeast Extract from Difco; the commercial product is retained without an ontology grounding.
8	Casamino acids	1.0 G_PER_L	FOODON:03315719 Casamino acids	TOGO M2040 and NBRC Medium 1338 list 1.0 g/L Casamino acids.
9	Bacto Proteose Peptone No. 3 (Difco)	3.0 G_PER_L	MICRO:0000180 (source link unavailable) Proteose Peptone	TOGO M2040 and NBRC Medium 1338 list 3.0 g/L Bacto Proteose Peptone No. 3 from Difco.

Preparation

Adjust pH to 7.0-7.2.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:58.939490Z	togo-import	Imported from TOGO Medium	Source: NBRC, ID: M2040
2026-01-28T19:48:14.165019Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:58.862536Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008630
2026-03-17T06:29:55.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.485403+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "NSSM medium" to "nssm_medium" for consistent matching
2026-06-05T07:41:40.092991+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.431504+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:09.693637+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00	repair_nutrient_ncg_nssm_score15.py	RESOLVED_TOGO_M2040_SCORE15	TOGO M2040 imports NBRC Medium 1338 NSSM medium. The NBRC source lists 132 g NaCl, 0.6 g K2HPO4, 0.05 g sodium pyruvate, 1 L artificial seawater, 1 g glucose, 20 g agar, 1 g Bacto Yeast Extract, 1 g Casamino acids, and 3 g Bacto Proteose Peptone No. 3; pH is 7.0-7.2.