Overview
- External ontology
- TOGO:M2065 — AF6+Rice Extract
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2065 Original source: NBRC - NBRC_M1372 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1372
Composition
flowchart LR
CultureMech_008655["af6_rice_extract"]
ing0_Distilled_water["Distilled water"]
CultureMech_008655 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008655 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008655 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008655 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_008655 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Biotin["Biotin"]
CultureMech_008655 --> ing5_Biotin
CHEBI_15956["CHEBI:15956"]
ing5_Biotin -.-> CHEBI_15956
ing6_Thiamine_HCl["Thiamine-HCl"]
CultureMech_008655 --> ing6_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing6_Thiamine_HCl -.-> CHEBI_49105
ing7_Vitamin_B12["Vitamin B12"]
CultureMech_008655 --> ing7_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing7_Vitamin_B12 -.-> CHEBI_176843
ing8_NaNO3["NaNO3"]
CultureMech_008655 --> ing8_NaNO3
CHEBI_63005["CHEBI:63005"]
ing8_NaNO3 -.-> CHEBI_63005
ing9_CaCO3["CaCO3"]
CultureMech_008655 --> ing9_CaCO3
CHEBI_3311["CHEBI:3311"]
ing9_CaCO3 -.-> CHEBI_3311
ing10_NH4NO3["NH4NO3"]
CultureMech_008655 --> ing10_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing10_NH4NO3 -.-> CHEBI_63038
ing11_Fe_citrate["Fe-citrate"]
CultureMech_008655 --> ing11_Fe_citrate
ing12_MES["MES"]
CultureMech_008655 --> ing12_MES
CHEBI_39010["CHEBI:39010"]
ing12_MES -.-> CHEBI_39010
ing13_Agar__if_needed_["Agar (if needed)"]
CultureMech_008655 --> ing13_Agar__if_needed_
ing14_Vitamin_B6["Vitamin B6"]
CultureMech_008655 --> ing14_Vitamin_B6
CHEBI_16709["CHEBI:16709"]
ing14_Vitamin_B6 -.-> CHEBI_16709
ing15_Citric_acid["Citric acid"]
CultureMech_008655 --> ing15_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing15_Citric_acid -.-> CHEBI_30769
ing16_Rice_Extract["Rice Extract"]
CultureMech_008655 --> ing16_Rice_Extract
ing17_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008655 --> ing17_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_2H2O -.-> CHEBI_75213
ing18_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008655 --> ing18_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_4H2O -.-> CHEBI_86368
ing19_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008655 --> ing19_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing19_CoCl2_6H2O -.-> CHEBI_35696
ing20_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008655 --> ing20_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_7H2O -.-> CHEBI_32312
ing21_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008655 --> ing21_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing21_FeCl3_6H2O -.-> CHEBI_86254
ing22_Na2EDTA_2H2O["Na2EDTA·2H2O"]
CultureMech_008655 --> ing22_Na2EDTA_2H2O
CHEBI_64758["CHEBI:64758"]
ing22_Na2EDTA_2H2O -.-> CHEBI_64758
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
30 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
10 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
10 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 7 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
10 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 8 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 9 |
NaNO3
NO3.Na 84.994 g/mol
|
140 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaCO3
CO3.Ca 100.086 g/mol
|
10 G_PER_L |
CHEBI:3311
calcium carbonate
|
Role: Mineral source, Protective agent; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NH4NO3
H4N.NO3 80.043 g/mol
|
22 G_PER_L |
CHEBI:63038
ammonium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | Fe-citrate | 2 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 13 | MES | 400 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 14 | Agar (if needed) | 12 G_PER_L | Role: Solidifying component | |
| 15 |
Vitamin B6
C8H11NO3 169.18 g/mol
|
1 G_PER_L |
CHEBI:16709
pyridoxine
|
Role: Vitamin |
| 16 |
Citric acid
C6H8O7 192.123 g/mol
|
2 G_PER_L |
CHEBI:30769
citric acid
|
Properties: Defined component, Organic compound, Simple component |
| 17 | Rice Extract | variable VARIABLE | Properties: Undefined component, Simple component | |
| 18 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.25 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
3.6 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.4 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
2.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
19.6 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2EDTA·2H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
100 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
Properties: Defined component, Simple component, Organic compound |
Solutions
P IV Metals solution*
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- af6_rice_extract (CultureMech:008655)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.988852Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2065 |
| 2026-01-28T19:48:29.655304Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Rice Extract |
| 2026-03-09T08:04:49.268319+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:20.411837Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:59.098871Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008655 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.726500+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AF6+Rice Extract" to "af6_rice_extract" for consistent matching |
| 2026-05-16T09:03:23.446057+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:47.584534+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:35.345932+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.695209+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.160715+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d16f298c55a52d84fb6a751ffa5832284d72cfafb5c07213d5a5e21dc7ef84f5 |