af6_rice_extract (CultureMech:008655)

Overview

External ontology

TOGO:M2065 — AF6+Rice Extract

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2065 Original source: NBRC - NBRC_M1372 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1372

Composition

flowchart LR
    CultureMech_008655["af6_rice_extract"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008655 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008655 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008655 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008655 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_008655 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Biotin["Biotin"]
    CultureMech_008655 --> ing5_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing5_Biotin -.-> CHEBI_15956
    ing6_Thiamine_HCl["Thiamine-HCl"]
    CultureMech_008655 --> ing6_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing6_Thiamine_HCl -.-> CHEBI_49105
    ing7_Vitamin_B12["Vitamin B12"]
    CultureMech_008655 --> ing7_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing7_Vitamin_B12 -.-> CHEBI_176843
    ing8_NaNO3["NaNO3"]
    CultureMech_008655 --> ing8_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing8_NaNO3 -.-> CHEBI_63005
    ing9_CaCO3["CaCO3"]
    CultureMech_008655 --> ing9_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing9_CaCO3 -.-> CHEBI_3311
    ing10_NH4NO3["NH4NO3"]
    CultureMech_008655 --> ing10_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing10_NH4NO3 -.-> CHEBI_63038
    ing11_Fe_citrate["Fe-citrate"]
    CultureMech_008655 --> ing11_Fe_citrate
    ing12_MES["MES"]
    CultureMech_008655 --> ing12_MES
    CHEBI_39010["CHEBI:39010"]
    ing12_MES -.-> CHEBI_39010
    ing13_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008655 --> ing13_Agar__if_needed_
    ing14_Vitamin_B6["Vitamin B6"]
    CultureMech_008655 --> ing14_Vitamin_B6
    CHEBI_16709["CHEBI:16709"]
    ing14_Vitamin_B6 -.-> CHEBI_16709
    ing15_Citric_acid["Citric acid"]
    CultureMech_008655 --> ing15_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing15_Citric_acid -.-> CHEBI_30769
    ing16_Rice_Extract["Rice Extract"]
    CultureMech_008655 --> ing16_Rice_Extract
    ing17_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008655 --> ing17_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_2H2O -.-> CHEBI_75213
    ing18_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008655 --> ing18_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_4H2O -.-> CHEBI_86368
    ing19_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008655 --> ing19_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing19_CoCl2_6H2O -.-> CHEBI_35696
    ing20_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_008655 --> ing20_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_7H2O -.-> CHEBI_32312
    ing21_FeCl3_6H2O["FeCl3·6H2O"]
    CultureMech_008655 --> ing21_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing21_FeCl3_6H2O -.-> CHEBI_86254
    ing22_Na2EDTA_2H2O["Na2EDTA·2H2O"]
    CultureMech_008655 --> ing22_Na2EDTA_2H2O
    CHEBI_64758["CHEBI:64758"]
    ing22_Na2EDTA_2H2O -.-> CHEBI_64758

Ingredients (23)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	101.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 100.0]
2	MgSO4·7H2O 7H2O.Mg.O4S 246.473 g/mol	30 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	CaCl2·2H2O Ca.2Cl.2H2O 147.014 g/mol	10 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	KH2PO4 H2O4P.K 136.084 g/mol	10 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5	K2HPO4 HO4P.2K 174.174 g/mol	5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6	Biotin C10H16N2O3S 244.316 g/mol	2 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
7	Thiamine-HCl C12H17N4OS.Cl.HCl 337.276 g/mol	10 G_PER_L	CHEBI:49105 thiamine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
8	Vitamin B12	1 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
9	NaNO3 NO3.Na 84.994 g/mol	140 G_PER_L	CHEBI:63005 sodium nitrate	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
10	CaCO3 CO3.Ca 100.086 g/mol	10 G_PER_L	CHEBI:3311 calcium carbonate	Role: Mineral source, Protective agent; Properties: Inorganic compound, Defined component, Simple component
11	NH4NO3 H4N.NO3 80.043 g/mol	22 G_PER_L	CHEBI:63038 ammonium nitrate	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
12	Fe-citrate	2 G_PER_L		Role: Mineral source; Properties: Organic compound, Defined component, Simple component
13	MES	400 G_PER_L	CHEBI:39010 MES	Role: Buffer; Properties: Organic compound, Defined component, Simple component
14	Agar (if needed)	12 G_PER_L		Role: Solidifying component
15	Vitamin B6 C8H11NO3 169.18 g/mol	1 G_PER_L	CHEBI:16709 pyridoxine	Role: Vitamin
16	Citric acid C6H8O7 192.123 g/mol	2 G_PER_L	CHEBI:30769 citric acid	Properties: Defined component, Organic compound, Simple component
17	Rice Extract	variable VARIABLE		Properties: Undefined component, Simple component
18	Na2MoO4·2H2O 2H2O.MoO4.2Na 241.946 g/mol	0.25 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19	MnCl2·4H2O Cl2Mn.4H2O 197.904 g/mol	3.6 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
20	CoCl2·6H2O 2Cl.Co 129.839 g/mol	0.4 G_PER_L	CHEBI:35696 cobalt dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21	ZnSO4·7H2O 7H2O.O4S.Zn 287.558 g/mol	2.2 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22	FeCl3·6H2O Cl3Fe.6H2O 270.294 g/mol	19.6 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23	Na2EDTA·2H2O C10H14N2O8.2H2O.2Na 372.238 g/mol	100 G_PER_L	CHEBI:64758 EDTA disodium salt dihydrate	Properties: Defined component, Simple component, Organic compound

Solutions

P IV Metals solution*

Dose: 5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:58.988852Z	togo-import	Imported from TOGO Medium	Source: NBRC, ID: M2065
2026-01-28T19:48:29.655304Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Rice Extract
2026-03-09T08:04:49.268319+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T03:09:20.411837Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:19:59.098871Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008655
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:28.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.726500+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "AF6+Rice Extract" to "af6_rice_extract" for consistent matching
2026-05-16T09:03:23.446057+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:47.584534+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:35.345932+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:52.695209+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.