Overview
- External ontology
- TOGO:M213 — SC Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M213 Original source: JCM - JCM_M220 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=220 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008734["sc_medium"]
ing0_50__Glucose["50% Glucose"]
CultureMech_008734 --> ing0_50__Glucose
ing1_10__Cysteine["10% Cysteine"]
CultureMech_008734 --> ing1_10__Cysteine
ing2_Tryptone["Tryptone"]
CultureMech_008734 --> ing2_Tryptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_008734 --> ing3_Yeast_extract
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_008734 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Distilled_water["Distilled water"]
CultureMech_008734 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
ing6_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008734 --> ing6_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_7H2O -.-> CHEBI_31795
ing7_KH2PO4["KH2PO4"]
CultureMech_008734 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_K2HPO4["K2HPO4"]
CultureMech_008734 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_Hemin__0_1__in_0_05_N_NaOH_["Hemin (0.1% in 0.05 N NaOH)"]
CultureMech_008734 --> ing9_Hemin__0_1__in_0_05_N_NaOH_
ing10_Corn_meal_agar__BD_Difco_["Corn meal agar (BD-Difco)"]
CultureMech_008734 --> ing10_Corn_meal_agar__BD_Difco_
ing11_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
CultureMech_008734 --> ing11_Phytone_peptone__BD_BBL_
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 50% Glucose | 1 G_PER_L | Role: Carbon source; Properties: Defined component, Organic compound, Solution | |
| 2 | 10% Cysteine | 10 G_PER_L | Role: Nitrogen source; Properties: Defined component, Organic compound, Solution | |
| 3 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 4 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 6 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 10 | Hemin (0.1% in 0.05 N NaOH) | 15 G_PER_L | Role: Growth factor; Properties: Defined component, Organic compound, Solution | |
| 11 | Corn meal agar (BD-Difco) | 17 G_PER_L | Properties: Undefined component, Complex component | |
| 12 | Phytone peptone (BD-BBL) | 8 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M213_SC_Medium (CultureMech:008734)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.626929Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M213 |
| 2026-01-28T19:48:23.035916Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:59.922965Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008734 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.570379+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SC Medium" to "sc_medium" for consistent matching |
| 2026-06-05T07:40:35.521510+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:25.512644+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.616540+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:10.479955+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bb5cb0f5388b8f85243b57d7e49fcd361d57ebd2de4f3afa0fcfe2502906786d |