sc_medium

CultureMech:008734 bacterial COMPLEX SOLID_AGAR
Original name: SC Medium

Overview

External ontology
TOGO:M213 — SC Medium
Applications
  • Microbial cultivation
pH
6.6
Notes
Source: https://togomedium.org/medium/M213 Original source: JCM - JCM_M220 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=220 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The exact recipe specifies albumin, not LB broth. Remove only the three reviewed LB supplier nodes and restore albumin at its source-defined preparation stage. Genuine yeast extract and unrelated annotations are preserved.

Composition

flowchart LR
    CultureMech_008734["sc_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008734 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_008734 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_KH2PO4["KH2PO4"]
    CultureMech_008734 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_K2HPO4["K2HPO4"]
    CultureMech_008734 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Corn_meal_agar__BD_Difco_["Corn meal agar (BD-Difco)"]
    CultureMech_008734 --> ing4_Corn_meal_agar__BD_Difco_
    ing5_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
    CultureMech_008734 --> ing5_Phytone_peptone__BD_BBL_

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component Source water batch input: 1 L = 1 L. This is not a measured final volume.
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound Source batch input: 0.2 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
3 KH2PO4
H2O4P.K 136.084 g/mol
CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Defined component, Inorganic compound Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
4 K2HPO4
HO4P.2K 174.174 g/mol
CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
5 Corn meal agar (BD-Difco) Properties: Undefined component, Complex component Source batch input: 17 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
6 Phytone peptone (BD-BBL) Properties: Undefined component Source batch input: 8 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.

Preparation

  1. Adjust pH to 6.6.
  2. Separately sterilize bovine serum albumin, glucose and cysteine solutions by filtration, and aseptically add to the medium.

Solutions

50% Glucose

Dose: 0.001 L

Stock composition
IngredientConcentrationNotes
Glucose Source preparation label: 50% Glucose. Percentage basis is unspecified; no numeric stock strength, stock-batch mass or final-medium concentration is asserted. The enclosing descriptor holds the batch volume.
Role: Carbon source; Properties: Defined component, Organic compound, Solution Source batch dose: 1 ml = 0.001 L per receiving batch, not g/L or a dose per final liter.

10% Cysteine

Dose: 0.01 L

Stock composition
IngredientConcentrationNotes
Cysteine Source preparation label: 10% Cysteine. Percentage basis is unspecified; no numeric stock strength, stock-batch mass or final-medium concentration is asserted. The enclosing descriptor holds the batch volume.
Role: Nitrogen source; Properties: Defined component, Organic compound, Solution Source batch dose: 10 ml = 0.01 L per receiving batch, not g/L or a dose per final liter.

20% Bovine serum albumin, fraction V

Dose: 0.01 L

Stock composition
IngredientConcentrationNotes
Bovine serum albumin, fraction V Source preparation label: 20% Bovine serum albumin, fraction V. Percentage basis is unspecified; no numeric stock strength, stock-batch mass or final-medium concentration is asserted. The enclosing descriptor holds the batch volume.
Source batch dose: 10 ml = 0.01 L per receiving batch, not g/L or a dose per final liter.

Hemin (0.1% in 0.05 N NaOH)

Dose: 0.015 L

Stock composition
IngredientConcentrationNotes
Hemin Source preparation label: Hemin (0.1% in 0.05 N NaOH). Percentage basis is unspecified; no numeric stock strength, stock-batch mass or final-medium concentration is asserted. The enclosing descriptor holds the batch volume.
Role: Growth factor; Properties: Defined component, Organic compound, Solution Source batch dose: 15 ml = 0.015 L per receiving batch, not g/L or a dose per final liter.

NaOH solution (0.05 N)

Stock composition
IngredientConcentrationNotes
NaOH Hemin-stock solvent is 0.05 N NaOH. No solvent dose or stock preparation volume is supplied; normality is not converted to molarity. The source does not assign the separate three-stock filtration instruction to hemin.
Hemin-stock solvent is 0.05 N NaOH. No solvent dose or stock preparation volume is supplied; normality is not converted to molarity. The source does not assign the separate three-stock filtration instruction to hemin.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.626929Z togo-import Imported from TOGO Medium Source: JCM, ID: M213
2026-01-28T19:48:23.035916Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:59.922965Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008734
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.570379+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SC Medium" to "sc_medium" for consistent matching
2026-06-05T07:40:35.521510+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:25.512644+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:48.616540+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-08T21:45:30.677002+00:00 codex RESTORED_ALBUMIN_COUNTERPART_IDENTITIES_AND_STOCK_CONCENTRATIONS_WITH_EVIDENCE Restore albumin and remove false LB constituents; correct mass/dose and stock/receiving-batch scopes. Retain exact source batch masses without assumed final volume. Unspecified percentage bases remain unknown. No growth claims or new chemical IDs are inferred.