nutrient_broth_no_2

CultureMech:008765 bacterial COMPLEX SOLID_AGAR
Original name: Nutrient broth No.2

Overview

External ontology
TOGO:M2170 — Nutrient broth No.2
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2170 Original source: NBRC - NBRC_M1559 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1559

Composition

flowchart LR
    CultureMech_008765["nutrient_broth_no_2"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008765 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Sodium_chloride["Sodium chloride"]
    CultureMech_008765 --> ing1_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing1_Sodium_chloride -.-> CHEBI_26710
    ing2_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008765 --> ing2_Agar__if_needed_
    ing3_Lab_Lemco_powder["Lab-Lemco powder"]
    CultureMech_008765 --> ing3_Lab_Lemco_powder
    ing4_Peptone["Peptone"]
    CultureMech_008765 --> ing4_Peptone

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Sodium chloride
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 Agar (if needed) 15 G_PER_L Role: Solidifying component
4 Lab-Lemco powder 10 G_PER_L Properties: Undefined component
5 Peptone 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:59.199998Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2170
2026-01-28T19:48:38.602575Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.132790Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008765
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.793423+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient broth No.2" to "nutrient_broth_no_2" for consistent matching
2026-06-05T07:41:40.184759+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:51.725694+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 25d3424c5e25b603ef574eedd42c2846c2179737c09427f2bca0501184ca6c5e