nutrient_broth_no_2

CultureMech:008765 bacterial COMPLEX SOLID_AGAR
Original name: Nutrient broth No.2

Overview

External ontology
TOGO:M2170 — Nutrient broth No.2
Applications
  • Microbial cultivation
pH range
{'min': 7.3, 'max': 7.7}
Notes
TOGO M2170 imports NBRC Medium 1559 Nutrient broth No. 2: 10 g Lab-Lemco powder, 10 g peptone, 5 g sodium chloride, 15 g agar if needed, and 1 L distilled water; pH is 7.5 +/- 0.2.

Composition

flowchart LR
    CultureMech_008765["nutrient_broth_no_2"]
    ing0_Lab_Lemco_powder["Lab-Lemco powder"]
    CultureMech_008765 --> ing0_Lab_Lemco_powder
    ing1_Peptone["Peptone"]
    CultureMech_008765 --> ing1_Peptone
    ing2_Sodium_chloride["Sodium chloride"]
    CultureMech_008765 --> ing2_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_chloride -.-> CHEBI_26710
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008765 --> ing3_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_needed_ -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_008765 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Lab-Lemco powder 10.0 G_PER_L FOODON:03302088
Beef extract
 TOGO M2170 and NBRC Medium 1559 list 10.0 g/L Lab-Lemco powder.
2 Peptone 10.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 TOGO M2170 and NBRC Medium 1559 list 10.0 g/L Peptone.
3 Sodium chloride
Cl.Na 58.443 g/mol
5.0 G_PER_L CHEBI:26710
sodium chloride
 TOGO M2170 and NBRC Medium 1559 list 5.0 g/L Sodium chloride.
4 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M2170 and NBRC Medium 1559 list 15.0 g/L Agar if needed for solid medium.
5 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 TOGO M2170 and NBRC Medium 1559 list 1 L Distilled water.

Preparation

  1. Adjust pH to 7.5 +/- 0.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:59.199998Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2170
2026-01-28T19:48:38.602575Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.132790Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008765
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.793423+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient broth No.2" to "nutrient_broth_no_2" for consistent matching
2026-06-05T07:41:40.184759+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_nutrient_broths_score15.py RESOLVED_TOGO_M2170_NBRC_1559 TOGO M2170 imports NBRC Medium 1559 Nutrient broth No. 2: 10 g Lab-Lemco powder, 10 g peptone, 5 g sodium chloride, 15 g agar if needed, and 1 L distilled water; pH is 7.5 +/- 0.2.