Overview
- External ontology
- TOGO:M2181 — SMC medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2181 Original URL: https://www.atcc.org/~/media/6ACA1A2B1C7A47EFA575372A421E1E77.ashx
Composition
flowchart LR
CultureMech_008776["smc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008776 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Sorbitol["Sorbitol"]
CultureMech_008776 --> ing1_Sorbitol
CHEBI_30911["CHEBI:30911"]
ing1_Sorbitol -.-> CHEBI_30911
ing2_Tryptone__BD_211705_["Tryptone (BD 211705)"]
CultureMech_008776 --> ing2_Tryptone__BD_211705_
ing3_PPLO_Broth_w_o_CV__Mycoplasma_Broth___BD_255420_["PPLO Broth w/o CV (Mycoplasma Broth) (BD 255420)"]
CultureMech_008776 --> ing3_PPLO_Broth_w_o_CV__Mycoplasma_Broth___BD_255420_
ing4_42__L_Arginine_HCl["42% L-Arginine HCl"]
CultureMech_008776 --> ing4_42__L_Arginine_HCl
ing5_Horse_serum["Horse serum"]
CultureMech_008776 --> ing5_Horse_serum
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
650 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Sorbitol
C6H14O6 182.172 g/mol
|
70 G_PER_L |
CHEBI:30911
glucitol
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 3 | Tryptone (BD 211705) | 10 G_PER_L | Properties: Undefined component | |
| 4 | PPLO Broth w/o CV (Mycoplasma Broth) (BD 255420) | 21 G_PER_L | Properties: Undefined component, Complex component | |
| 5 | 42% L-Arginine HCl | 10 G_PER_L | Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component | |
| 6 | Horse serum | 200 G_PER_L | Role: Nutrient source; Properties: Undefined component |
Solutions
0.1% Phenol red solution
Dose: 20 G_PER_L
Role: pH indicator; Properties: Solution, Organic compound, Defined component
50% Fructose solution
Dose: 2 G_PER_L
Role: Carbon source; Properties: Defined component, Solution, Organic compound
50% Glucose solution
Dose: 2 G_PER_L
Role: Carbon source; Properties: Defined component, Solution, Organic compound
50% Sucrose solution
Dose: 20 G_PER_L
Role: Carbon source
Yeast Extract Solution (GIBCO 360-8180)
Dose: 100 G_PER_L
Role: Nutrient source; Properties: Undefined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- smc_medium (CultureMech:008776)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.132100Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2181 |
| 2026-01-28T19:48:20.129968Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:33.384116+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T06:20:00.220308Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008776 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.885127+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SMC medium" to "smc_medium" for consistent matching |
| 2026-05-16T09:04:31.774159+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:45.936532+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:55.818952+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 92d232dc8da4c2652008847050202fa9fb9b38f013212db4876cad403bbaee57 |