smc_medium (CultureMech:008776)

Overview

External ontology

TOGO:M2181 — SMC medium

Applications

Microbial cultivation

pH

7.5

Notes

TOGO M2181 cites ATCC Medium 668 SMC medium. ATCC Medium 668 lists a basal recipe with 21.0 g PPLO Broth w/o CV from BD 255420, 10.0 g Tryptone from BD 211705, 70.0 g Sorbitol, and 650.0 ml Distilled water; the pH is adjusted to 7.5 and the base is autoclaved at 121 C for 15 minutes. The source then filter-sterilizes and aseptically adds 200.0 ml Horse serum, 100.0 ml Yeast Extract Solution from GIBCO 360-8180, 2.0 ml 50% Fructose solution, 20.0 ml 50% Sucrose solution, 2.0 ml 50% Glucose solution, 10.0 ml 42% L-Arginine HCl, and 20.0 ml 0.1% Phenol red solution.

Composition

flowchart LR
    CultureMech_008776["smc_medium"]
    ing0_PPLO_Broth_w_o_CV__Mycoplasma_Broth___BD_255420_["PPLO Broth w/o CV (Mycoplasma Broth) (BD 255420)"]
    CultureMech_008776 --> ing0_PPLO_Broth_w_o_CV__Mycoplasma_Broth___BD_255420_
    ing1_Tryptone__BD_211705_["Tryptone (BD 211705)"]
    CultureMech_008776 --> ing1_Tryptone__BD_211705_
    ing2_Sorbitol["Sorbitol"]
    CultureMech_008776 --> ing2_Sorbitol
    CHEBI_30911["CHEBI:30911"]
    ing2_Sorbitol -.-> CHEBI_30911
    ing3_Distilled_water["Distilled water"]
    CultureMech_008776 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_Horse_serum["Horse serum"]
    CultureMech_008776 --> ing4_Horse_serum

Ingredients (5)

#	Ingredient	Concentration	Ontology	Notes
1	PPLO Broth w/o CV (Mycoplasma Broth) (BD 255420)	21.0 G_PER_L		TOGO M2181 / ATCC Medium 668 lists 21.0 g PPLO Broth w/o CV from BD catalog 255420; the commercial broth is retained as an opaque complex component.
2	Tryptone (BD 211705)	10.0 G_PER_L	MICRO:0000182 (source link unavailable) Tryptone	TOGO M2181 / ATCC Medium 668 lists 10.0 g/L Tryptone (BD 211705).
3	Sorbitol C6H14O6 182.172 g/mol	70.0 G_PER_L	CHEBI:30911 glucitol	TOGO M2181 / ATCC Medium 668 lists 70.0 g/L Sorbitol.
4	Distilled water H2O 18.015 g/mol	650.0 ML_PER_L	CHEBI:15377 water	TOGO M2181 / ATCC Medium 668 lists 650.0 ml/L Distilled water.
5	Horse serum	200.0 ML_PER_L	MICRO:0001235 (source link unavailable) Horse serum	TOGO M2181 / ATCC Medium 668 lists 200.0 ml horse serum; this serum is retained as an opaque complex component.

Preparation

Mix PPLO Broth w/o CV, Tryptone, Sorbitol, and Distilled water as the basal medium.
Adjust the basal medium to pH 7.5.
Autoclave the basal medium at 121 C for 15 minutes.
Filter-sterilize the serum, yeast extract, sugar, arginine HCl, and phenol red additions.
Aseptically add the filtered stocks to the sterile basal medium.

Solutions

Yeast Extract Solution (GIBCO 360-8180)

Dose: 100.0 ML_PER_L

TOGO M2181 / ATCC Medium 668 lists 100.0 ml Yeast Extract Solution (GIBCO 360-8180); this GIBCO yeast extract solution is retained as an opaque complex component because the ATCC source does not disclose its stock composition.

50% Fructose solution

Dose: 2.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Fructose	50.0 PERCENT_W_V	TOGO M2181 / ATCC Medium 668 identifies 50% Fructose solution as a 50.0 % w/v Fructose stock.

TOGO M2181 / ATCC Medium 668 aseptically adds 2.0 ml/L 50% Fructose solution.

50% Sucrose solution

Dose: 20.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Sucrose	50.0 PERCENT_W_V	TOGO M2181 / ATCC Medium 668 identifies 50% Sucrose solution as a 50.0 % w/v Sucrose stock.

TOGO M2181 / ATCC Medium 668 aseptically adds 20.0 ml/L 50% Sucrose solution.

50% Glucose solution

Dose: 2.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Glucose	50.0 PERCENT_W_V	TOGO M2181 / ATCC Medium 668 identifies 50% Glucose solution as a 50.0 % w/v Glucose stock.

TOGO M2181 / ATCC Medium 668 aseptically adds 2.0 ml/L 50% Glucose solution.

42% L-Arginine HCl

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
L-Arginine HCl	42.0 PERCENT_W_V	TOGO M2181 / ATCC Medium 668 identifies 42% L-Arginine HCl as a 42.0 % w/v L-Arginine HCl stock.

TOGO M2181 / ATCC Medium 668 aseptically adds 10.0 ml/L 42% L-Arginine HCl.

0.1% Phenol red solution

Dose: 20.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Phenol red	0.1 PERCENT_W_V	TOGO M2181 / ATCC Medium 668 identifies 0.1% Phenol red solution as a 0.1 % w/v Phenol red stock.

TOGO M2181 / ATCC Medium 668 aseptically adds 20.0 ml/L 0.1% Phenol red solution.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.132100Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2181
2026-01-28T19:48:20.129968Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:33.384116+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 5 solution(s) from ingredients
2026-03-13T06:20:00.220308Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008776
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:08.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.885127+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "SMC medium" to "smc_medium" for consistent matching
2026-05-16T09:04:31.774159+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:41:45.936532+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00	repair_togo_m2181_smc_score15.py	RESOLVED_TOGO_M2181_SMC_SCORE15	TOGO M2181 cites ATCC Medium 668 SMC medium. ATCC Medium 668 lists a basal recipe with 21.0 g PPLO Broth w/o CV from BD 255420, 10.0 g Tryptone from BD 211705, 70.0 g Sorbitol, and 650.0 ml Distilled water; the pH is adjusted to 7.5 and the base is autoclaved at 121 C for 15 minutes. The source then filter-sterilizes and aseptically adds 200.0 ml Horse serum, 100.0 ml Yeast Extract Solution from GIBCO 360-8180, 2.0 ml 50% Fructose solution, 20.0 ml 50% Sucrose solution, 2.0 ml 50% Glucose solution, 10.0 ml 42% L-Arginine HCl, and 20.0 ml 0.1% Phenol red solution. Corrected the imported ml additions, added the ATCC pH and sterilization instructions, moved disclosed stocks into solutions with compositions, and kept PPLO broth, horse serum, and the GIBCO yeast extract solution as curated opaque inputs.