Overview
- External ontology
- TOGO:M2215 — PPLO broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2215
Composition
flowchart LR
CultureMech_008805["pplo_broth"]
ing0_1___w_v__Phenol_red["1% (w/v) Phenol red"]
CultureMech_008805 --> ing0_1___w_v__Phenol_red
ing1_25___w_v__Glucose["25% (w/v) Glucose"]
CultureMech_008805 --> ing1_25___w_v__Glucose
ing2_Distilled_water["Distilled water"]
CultureMech_008805 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_Horse_serum["Horse serum"]
CultureMech_008805 --> ing3_Horse_serum
ing4_Ampicillin["Ampicillin"]
CultureMech_008805 --> ing4_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing4_Ampicillin -.-> CHEBI_28971
ing5_Salmon_sperm_DNA__Sigma_["Salmon sperm DNA (Sigma)"]
CultureMech_008805 --> ing5_Salmon_sperm_DNA__Sigma_
ing6_PPLO_broth_powder__Difco_["PPLO broth powder (Difco)"]
CultureMech_008805 --> ing6_PPLO_broth_powder__Difco_
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 1% (w/v) Phenol red | 4 G_PER_L | Role: pH indicator; Properties: Defined component, Organic compound, Solution | |
| 2 | 25% (w/v) Glucose | 10 G_PER_L | Role: Carbon source; Properties: Defined component, Solution, Organic compound | |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 4 | Horse serum | 200 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
120 G_PER_L |
CHEBI:28971
ampicillin
|
Role: Antimicrobial agent; Properties: Simple component, Defined component, Organic compound |
| 6 | Salmon sperm DNA (Sigma) | 0.2 G_PER_L | Role: Growth factor; Properties: Undefined component | |
| 7 | PPLO broth powder (Difco) | 22.5 G_PER_L | Properties: Undefined component, Complex component |
Solutions
IsoVitaleX solution (Becton Dickinson)
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2215_PPLO_broth (CultureMech:008805)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.166956Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2215 |
| 2026-01-28T19:48:08.017886Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:12.888960+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:00.382648Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008805 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.056909+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPLO broth" to "pplo_broth" for consistent matching |
| 2026-05-16T09:03:12.549130+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.715016+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:10.716969+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 38d74b1a031702c618e9a868ff5bafbcc95ef094fb658fdee4d83902eed4df29 |