Overview
- External ontology
- TOGO:M2215 — PPLO broth
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2215 lists PPLO broth as a growth medium containing, per liter, 22.5 g PPLO broth powder from Difco, 0.2 g Salmon sperm DNA from Sigma, 200 ml horse serum, 5 ml IsoVitaleX solution from Becton Dickinson, 10 ml 25% (w/v) Glucose, 4 ml 1% (w/v) Phenol red, 120 mg Ampicillin, and 1 L distilled water. The TOGO record does not state pH, temperature, or sterilization conditions.
Composition
flowchart LR
CultureMech_008805["pplo_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_008805 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Horse_serum["Horse serum"]
CultureMech_008805 --> ing1_Horse_serum
ing2_Ampicillin["Ampicillin"]
CultureMech_008805 --> ing2_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing2_Ampicillin -.-> CHEBI_28971
ing3_Salmon_sperm_DNA__Sigma_["Salmon sperm DNA (Sigma)"]
CultureMech_008805 --> ing3_Salmon_sperm_DNA__Sigma_
ing4_PPLO_broth_powder__Difco_["PPLO broth powder (Difco)"]
CultureMech_008805 --> ing4_PPLO_broth_powder__Difco_
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M2215 lists 1 L distilled water. |
| 2 | Horse serum | 200.0 ML_PER_L | TOGO M2215 lists 200 ml/L horse serum; this serum is retained as an opaque complex component. | |
| 3 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
120.0 MG_PER_L |
CHEBI:28971
ampicillin
|
TOGO M2215 lists 120.0 mg/L Ampicillin. |
| 4 | Salmon sperm DNA (Sigma) | 0.2 G_PER_L | TOGO M2215 lists 0.2 g/L Salmon sperm DNA from Sigma; this commercial biological product is retained as an opaque complex component. | |
| 5 | PPLO broth powder (Difco) | 22.5 G_PER_L | TOGO M2215 lists 22.5 g/L PPLO broth powder from Difco; the catalog broth is not reducible to one ChEBI molecule. |
Solutions
1% (w/v) Phenol red
Dose: 4.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Phenol red | 1.0 PERCENT_W_V | TOGO M2215 identifies the stock as 1.0 % w/v Phenol red. |
TOGO M2215 lists 4 ml/L 1% (w/v) Phenol red.
25% (w/v) Glucose
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | 25.0 PERCENT_W_V | TOGO M2215 identifies the stock as 25.0 % w/v Glucose. |
TOGO M2215 lists 10 ml/L 25% (w/v) Glucose.
IsoVitaleX solution (Becton Dickinson)
Dose: 5.0 ML_PER_L
TOGO M2215 lists 5 ml/L IsoVitaleX solution from Becton Dickinson; this commercial enrichment is retained as an opaque complex component.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.166956Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2215 |
| 2026-01-28T19:48:08.017886Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:12.888960+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:00.382648Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008805 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.056909+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPLO broth" to "pplo_broth" for consistent matching |
| 2026-05-16T09:03:12.549130+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.715016+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m2215_score15.py | RESOLVED_TOGO_M2215_SCORE15 | TOGO M2215 lists PPLO broth as a growth medium containing, per liter, 22.5 g PPLO broth powder from Difco, 0.2 g Salmon sperm DNA from Sigma, 200 ml horse serum, 5 ml IsoVitaleX solution from Becton Dickinson, 10 ml 25% (w/v) Glucose, 4 ml 1% (w/v) Phenol red, 120 mg Ampicillin, and 1 L distilled water. The TOGO record does not state pH, temperature, or sterilization conditions. Corrected ml/L and mg/L import artifacts, moved the disclosed glucose and phenol-red stocks into solutions, and kept commercial or biological complex inputs unmapped. |