Overview
- External ontology
- TOGO:M2226 — PPLO broth without CV (pH 7.8) with horse serum (not inactivated) and yeast extract
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2226 Original URL: https://www.atcc.org/~/media/AD2BC56A0BA94572A40803C02D24D35A.ashx
Composition
flowchart LR
CultureMech_008814["pplo_broth_without_cv_ph_7_8_with_horse_serum_not_inactivated_and_y..."]
ing0_Distilled_water["Distilled water"]
CultureMech_008814 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Horse_Serum__not_inactivated_["Horse Serum (not inactivated)"]
CultureMech_008814 --> ing1_Horse_Serum__not_inactivated_
ing2_PPLO_Broth_w_o_CV["PPLO Broth w/o CV"]
CultureMech_008814 --> ing2_PPLO_Broth_w_o_CV
ing3_Fresh_Baker_s_Yeast_Extract["Fresh Baker’s Yeast Extract"]
CultureMech_008814 --> ing3_Fresh_Baker_s_Yeast_Extract
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
700 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Horse Serum (not inactivated) | 200 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 | PPLO Broth w/o CV | 21 G_PER_L | Properties: Undefined component, Complex component | |
| 4 | Fresh Baker’s Yeast Extract | 100 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pplo_broth_without_cv_ph_7_8_with_horse_serum_not_inactivated_and_yeast_extract (CultureMech:008814)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.178750Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2226 |
| 2026-01-28T19:48:38.401220Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.447061Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008814 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.121836+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPLO broth without CV (pH 7.8) with horse serum (not inactivated) and yeast extract" to "pplo_broth_without_cv_ph_7_8_with_horse_serum_not_inactivated_and_yeast_extract" for consistent matching |
| 2026-06-05T07:41:42.445633+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:53.440673+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 30ce3b1f9b074d7d4ec40d19b76e2ab5d8aaabc40cd8f023053f19457575e85b |