pplo_broth_without_cv_ph_7_8_with_horse_serum_not_inactivated_and_yeast_extract

CultureMech:008814 bacterial COMPLEX LIQUID
Original name: PPLO broth without CV (pH 7.8) with horse serum (not inactivated) and yeast extract

Overview

External ontology
TOGO:M2226 — PPLO broth without CV (pH 7.8) with horse serum (not inactivated) and yeast extract
Applications
  • Microbial cultivation
pH
7.8
Notes
TOGO M2226 cites ATCC Medium 247. ATCC Medium 247 lists PPLO broth without CV at pH 7.8 as 21.0 g PPLO Broth w/o CV from BD catalog 255420, 200.0 ml non-inactivated horse serum, 100.0 ml Fresh Baker’s Yeast Extract from GIBCO 18180, and 700.0 ml distilled water. The horse serum and yeast extract are added aseptically to a sterile PPLO broth and water basal medium.

Composition

flowchart LR
    CultureMech_008814["pplo_broth_without_cv_ph_7_8_with_horse_serum_not_inactivated_and_y..."]
    ing0_PPLO_broth["PPLO broth"]
    CultureMech_008814 --> ing0_PPLO_broth
    ing1_Distilled_water["Distilled water"]
    CultureMech_008814 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Horse_serum["Horse serum"]
    CultureMech_008814 --> ing2_Horse_serum
    ing3_Fresh_Baker_s_Yeast_Extract__GIBCO_18180_["Fresh Baker’s Yeast Extract (GIBCO 18180)"]
    CultureMech_008814 --> ing3_Fresh_Baker_s_Yeast_Extract__GIBCO_18180_

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 PPLO broth 21.0 G_PER_L ATCC Medium 247 lists 21.0 g PPLO Broth w/o CV from BD catalog 255420, with the commercial product itself specified at pH 7.8; this catalog broth is retained as an opaque complex component.
2 Distilled water
H2O 18.015 g/mol
700.0 ML_PER_L CHEBI:15377
water
 ATCC Medium 247 lists 700.0 ml distilled water.
3 Horse serum 200.0 ML_PER_L MICRO:0001235 (source link unavailable)
Horse serum
 ATCC Medium 247 lists 200.0 ml horse serum and specifies that it is not inactivated; this serum is retained as an opaque complex component.
4 Fresh Baker’s Yeast Extract (GIBCO 18180) 100.0 ML_PER_L FOODON:03315426
Yeast extract
 ATCC Medium 247 lists 100.0 ml Fresh Baker’s Yeast Extract from GIBCO 18180; the broad FOODON Yeast extract term captures the disclosed extract class, and the source does not disclose the stock concentration.

Preparation

  1. Prepare sterile basal medium containing PPLO Broth w/o CV and distilled water.
  2. Aseptically add horse serum and Fresh Baker’s Yeast Extract (GIBCO 18180).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.178750Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2226
2026-01-28T19:48:38.401220Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.447061Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008814
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.121836+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PPLO broth without CV (pH 7.8) with horse serum (not inactivated) and yeast extract" to "pplo_broth_without_cv_ph_7_8_with_horse_serum_not_inactivated_and_yeast_extract" for consistent matching
2026-06-05T07:41:42.445633+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2226_pplo_horse_yeast_score15.py RESOLVED_TOGO_M2226_PPLO_HORSE_YEAST_SCORE15 TOGO M2226 cites ATCC Medium 247. ATCC Medium 247 lists PPLO broth without CV at pH 7.8 as 21.0 g PPLO Broth w/o CV from BD catalog 255420, 200.0 ml non-inactivated horse serum, 100.0 ml Fresh Baker’s Yeast Extract from GIBCO 18180, and 700.0 ml distilled water. The horse serum and yeast extract are added aseptically to a sterile PPLO broth and water basal medium. Corrected the imported ml/L rows, added the source pH, grounded water, horse serum, and yeast extract, and kept the ATCC PPLO broth unmapped as an opaque complex input.