Overview
- External ontology
- TOGO:M2240 — Medium containing PPLO broth, calf serum, glucose, penicillin G, and Tris-HC1
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2240
Composition
flowchart LR
CultureMech_008829["medium_containing_pplo_broth_calf_serum_glucose_penicillin_g_and_tr..."]
ing0_Tris_HC1__pH_7_6_["Tris-HC1 (pH 7.6)"]
CultureMech_008829 --> ing0_Tris_HC1__pH_7_6_
ing1_Glucose["Glucose"]
CultureMech_008829 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Calf_serum__Gibco_["Calf serum (Gibco)"]
CultureMech_008829 --> ing2_Calf_serum__Gibco_
ing3_PPLO_broth__Difco_["PPLO broth (Difco)"]
CultureMech_008829 --> ing3_PPLO_broth__Difco_
ing4_Penicillin_G["Penicillin G"]
CultureMech_008829 --> ing4_Penicillin_G
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tris-HC1 (pH 7.6) | 50 MILLIMOLAR | Role: Buffer; Properties: Complex component, Defined component, Solution | |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
0.2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 3 | Calf serum (Gibco) | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 | PPLO broth (Difco) | 2.2 G_PER_L | Properties: Undefined component, Complex component | |
| 5 | Penicillin G | variable VARIABLE | Role: Antimicrobial agent; Properties: Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_containing_pplo_broth_calf_serum_glucose_penicillin_g_and_tris_hc1 (CultureMech:008829)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.209724Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2240 |
| 2026-01-28T19:48:35.266748Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Penicillin G |
| 2026-03-13T06:20:00.600079Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008829 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.276966+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium containing PPLO broth, calf serum, glucose, penicillin G, and Tris-HC1" to "medium_containing_pplo_broth_calf_serum_glucose_penicillin_g_and_tris_hc1" for consistent matching |
| 2026-05-16T09:04:19.771861+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:28.757194+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:42.916730+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a36d5e3265b319ffcac6052730c176936a2a28f0664861570aa240caa6ba5bc4 |