medium_containing_pplo_broth_calf_serum_glucose_penicillin_g_and_tris_hc1 (CultureMech:008829)

Overview

External ontology

TOGO:M2240 — Medium containing PPLO broth, calf serum, glucose, penicillin G, and Tris-HC1

Applications

Microbial cultivation

pH

7.6

Notes

TOGO M2240 reports a 24 h, 37 C Mycoplasma capricolum ATCC 27343 growth medium containing 2.2% w/v PPLO broth from Difco, 1% v/v calf serum from Gibco, 0.2% w/v glucose, 400 units(U)/ml Penicillin G, and 50 mM Tris-HC1 at pH 7.6. The TOGO metadata also records pH 7.6.

Composition

flowchart LR
    CultureMech_008829["medium_containing_pplo_broth_calf_serum_glucose_penicillin_g_and_tr..."]
    ing0_Tris_HC1__pH_7_6_["Tris-HC1 (pH 7.6)"]
    CultureMech_008829 --> ing0_Tris_HC1__pH_7_6_
    ing1_Glucose["Glucose"]
    CultureMech_008829 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Calf_serum__Gibco_["Calf serum (Gibco)"]
    CultureMech_008829 --> ing2_Calf_serum__Gibco_
    ing3_PPLO_broth__Difco_["PPLO broth (Difco)"]
    CultureMech_008829 --> ing3_PPLO_broth__Difco_
    ing4_Penicillin_G["Penicillin G"]
    CultureMech_008829 --> ing4_Penicillin_G
    CHEBI_51765["CHEBI:51765"]
    ing4_Penicillin_G -.-> CHEBI_51765

Ingredients (5)

#	Ingredient	Concentration	Ontology	Notes
1	Tris-HC1 (pH 7.6)	50.0 MILLIMOLAR		TOGO M2240 lists 50 mM Tris-HC1 (pH 7.6); this pH-specific buffer is retained without a single-molecule ontology grounding.
2	Glucose C6H12O6 180.156 g/mol	0.2 PERCENT_W_V	CHEBI:17234 glucose	TOGO M2240 lists 0.2% w/v Glucose.
3	Calf serum (Gibco)	1.0 PERCENT_V_V		TOGO M2240 lists 1% v/v Calf serum from Gibco; this liquid serum is retained as an opaque complex component.
4	PPLO broth (Difco)	2.2 PERCENT_W_V		TOGO M2240 lists 2.2% w/v PPLO broth from Difco; the commercial broth is not reducible to one ChEBI molecule.
5	Penicillin G C16H17N2O4S.Na 356.379 g/mol	variable VARIABLE	CHEBI:51765 benzylpenicillin sodium	TOGO M2240 lists 400 units(U)/ml Penicillin G, which is retained as a variable concentration because the schema has no unit for activity units.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.209724Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2240
2026-01-28T19:48:35.266748Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Penicillin G
2026-03-13T06:20:00.600079Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008829
2026-03-17T06:30:40.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.276966+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Medium containing PPLO broth, calf serum, glucose, penicillin G, and Tris-HC1" to "medium_containing_pplo_broth_calf_serum_glucose_penicillin_g_and_tris_hc1" for consistent matching
2026-05-16T09:04:19.771861+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:41:28.757194+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:02:49.918353+00:00	apply_mim_groundings.py	Adopted MIM published grounding	Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-12T00:00:00-07:00	repair_togo_m2240_pplo_calf_serum_score15.py	RESOLVED_TOGO_M2240_SCORE15	TOGO M2240 reports a 24 h, 37 C Mycoplasma capricolum ATCC 27343 growth medium containing 2.2% w/v PPLO broth from Difco, 1% v/v calf serum from Gibco, 0.2% w/v glucose, 400 units(U)/ml Penicillin G, and 50 mM Tris-HC1 at pH 7.6. The TOGO metadata also records pH 7.6. Corrected the imported percent-concentration units, added the source pH, kept the disclosed opaque products unmapped, retained Penicillin G as an activity-unit amount, and removed kg_microbe_match mediadive.medium:21 because MediaDive medium 21 is Sarcina Medium.