Overview
- External ontology
- TOGO:M2267 — Autoinducer Bioassay (AB) Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2267 Original URL: https://www.atcc.org/~/media/F0C5AC9D96C345049F8AA78CCC2AB46A.ashx
Composition
flowchart LR
CultureMech_008854["autoinducer_bioassay_ab_medium"]
ing0_Base_Medium["Base Medium"]
CultureMech_008854 --> ing0_Base_Medium
ing1_NaCl["NaCl"]
CultureMech_008854 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_MgSO4["MgSO4"]
CultureMech_008854 --> ing2_MgSO4
CHEBI_32599["CHEBI:32599"]
ing2_MgSO4 -.-> CHEBI_32599
ing3_DI_Water["DI Water"]
CultureMech_008854 --> ing3_DI_Water
ing4_Casamino_Acids["Casamino Acids"]
CultureMech_008854 --> ing4_Casamino_Acids
ing5_3N_NaOH["3N NaOH"]
CultureMech_008854 --> ing5_3N_NaOH
ing6_1M_Potassium_Phosphate__pH_7_0_["1M Potassium Phosphate (pH 7.0)"]
CultureMech_008854 --> ing6_1M_Potassium_Phosphate__pH_7_0_
ing7_0_1M_L_arginine["0.1M L-arginine"]
CultureMech_008854 --> ing7_0_1M_L_arginine
ing8_Glycerol["Glycerol"]
CultureMech_008854 --> ing8_Glycerol
CHEBI_17754["CHEBI:17754"]
ing8_Glycerol -.-> CHEBI_17754
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Base Medium | 970 G_PER_L | ||
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
17.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
MgSO4
Mg.O4S 120.368 g/mol
|
12.3 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 | DI Water | 970 G_PER_L | Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound | |
| 5 | Casamino Acids | 2 G_PER_L | Properties: Undefined component | |
| 6 | 3N NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 7 | 1M Potassium Phosphate (pH 7.0) | 10 G_PER_L | Role: Buffer; Properties: Defined component, Inorganic compound, Simple component | |
| 8 | 0.1M L-arginine | 10 G_PER_L | Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound | |
| 9 |
Glycerol
C3H8O3 92.094 g/mol
|
10 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
Solutions
Stock Solutions
Dose: 30 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- autoinducer_bioassay_ab_medium (CultureMech:008854)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.247991Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2267 |
| 2026-01-28T19:48:36.863041Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 3N NaOH |
| 2026-03-09T08:05:01.544130+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:00.781723Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008854 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.466003+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Autoinducer Bioassay (AB) Medium" to "autoinducer_bioassay_ab_medium" for consistent matching |
| 2026-05-16T09:03:25.182822+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:50.099549+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.933945+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 4 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:53.649935+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.460492+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0ff097c87495529e57af45d5db48a9d1da167ac97a53aa2aac3763100856f533 |