autoinducer_bioassay_ab_medium

CultureMech:008854 bacterial COMPLEX LIQUID
Original name: Autoinducer Bioassay (AB) Medium

Overview

External ontology
TOGO:M2267 — Autoinducer Bioassay (AB) Medium
Applications
  • Microbial cultivation
pH
7.5
Notes
TOGO M2267 imports ATCC Medium 2746, Autoinducer Bioassay (AB) Medium. ATCC prepares a 970 ml base from NaCl, MgSO4, Casamino Acids, and DI Water, adjusts the pH to 7.5 with 3 N NaOH, autoclaves the base at 121 C, cools it completely, and adds 10 ml each 1 M Potassium Phosphate (pH 7.0), 0.1 M L-arginine, and Glycerol from separate sterile stocks.

Composition

flowchart LR
    CultureMech_008854["autoinducer_bioassay_ab_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_008854 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4["MgSO4"]
    CultureMech_008854 --> ing1_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing1_MgSO4 -.-> CHEBI_32599
    ing2_Casamino_Acids["Casamino Acids"]
    CultureMech_008854 --> ing2_Casamino_Acids
    ing3_DI_Water["DI Water"]
    CultureMech_008854 --> ing3_DI_Water
    CHEBI_15377["CHEBI:15377"]
    ing3_DI_Water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
17.5 G_PER_L CHEBI:26710
sodium chloride
 ATCC Medium 2746 lists 17.5 g/L NaCl.
2 MgSO4
Mg.O4S 120.368 g/mol
12.3 G_PER_L CHEBI:32599
magnesium sulfate
 ATCC Medium 2746 lists 12.3 g/L MgSO4.
3 Casamino Acids 2.0 G_PER_L ATCC Medium 2746 lists 2.0 g/L Casamino Acids.
4 DI Water
H2O 18.015 g/mol
970.0 ML_PER_L CHEBI:15377
water
 ATCC Medium 2746 lists 970 ml DI Water in the base.

Preparation

  1. Dissolve NaCl, MgSO4, and Casamino Acids in DI Water.
  2. Bring the pH of the base solution to 7.5 with 3 N NaOH.
  3. Autoclave the base at 121 C.
  4. Cool the autoclaved base completely.
  5. Add 10.0 ml/L each 1 M Potassium Phosphate (pH 7.0), 0.1 M L-arginine, and Glycerol from separate sterile stocks.

Solutions

3 N NaOH

Dose: variable VARIABLE

Stock composition
IngredientConcentrationNotes
NaOH 3.0 MOLAR The source uses 3 N NaOH for pH adjustment; for this monovalent base the stock is represented as 3.0 M NaOH.
ATCC Medium 2746 adjusts the base to pH 7.5 with 3 N NaOH.

Potassium phosphate buffer

Dose: 10.0 ML_PER_L

ATCC Medium 2746 adds 10 ml/L 1 M Potassium Phosphate (pH 7.0) from a separate sterile stock; the KH2PO4/K2HPO4 ratio is not disclosed.

L-Arginine solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Arginine 0.1 MOLAR ATCC Medium 2746 lists 0.1 M L-arginine as a sterile stock.
ATCC Medium 2746 adds 10 ml/L 0.1 M L-arginine.

Glycerol

Dose: 10.0 ML_PER_L

ATCC Medium 2746 adds 10 ml/L Glycerol from a separate sterile stock.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.247991Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2267
2026-01-28T19:48:36.863041Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: 3N NaOH
2026-03-09T08:05:01.544130+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:00.781723Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008854
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.466003+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Autoinducer Bioassay (AB) Medium" to "autoinducer_bioassay_ab_medium" for consistent matching
2026-05-16T09:03:25.182822+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:50.099549+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:35.933945+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 4 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:53.649935+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00 repair_togo_m2267_autoinducer_bioassay_score15.py RESOLVED_TOGO_M2267_AUTOINDUCER_BIOASSAY_SCORE15 Corrected TOGO's imported Base Medium and Stock Solutions wrappers into ATCC's base recipe plus sterile stock additions, fixed ml rows imported as g/L, added pH 7.5 and source preparation steps, grounded NaOH and L-arginine stocks, and kept the pH 7.0 potassium phosphate buffer ratio intentionally unexpanded.