Overview
- External ontology
- TOGO:M2287 — Mitsuhashi-Maramorosch (M&M) medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2287 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008873["mitsuhashi_maramorosch_m_m_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008873 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_yeast_extract["yeast extract"]
CultureMech_008873 --> ing1_yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008873 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_D_____glucose["D (+) glucose"]
CultureMech_008873 --> ing3_D_____glucose
ing4_KCl["KCl"]
CultureMech_008873 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaHCO3["NaHCO3"]
CultureMech_008873 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_CaCl2["CaCl2"]
CultureMech_008873 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_MgCl2["MgCl2"]
CultureMech_008873 --> ing7_MgCl2
CHEBI_6636["CHEBI:6636"]
ing7_MgCl2 -.-> CHEBI_6636
ing8_NaH2PO4["NaH2PO4"]
CultureMech_008873 --> ing8_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing8_NaH2PO4 -.-> CHEBI_37585
ing9_heat_inactivated_fetal_bovine_serum["heat-inactivated fetal bovine serum"]
CultureMech_008873 --> ing9_heat_inactivated_fetal_bovine_serum
ing10_Tryptone["Tryptone"]
CultureMech_008873 --> ing10_Tryptone
ing11_Yeast_extract["Yeast extract"]
CultureMech_008873 --> ing11_Yeast_extract
ing12_Sodium_chloride["Sodium chloride"]
CultureMech_008873 --> ing12_Sodium_chloride
ing12_Sodium_chloride -.-> CHEBI_26710
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component |
| 2 | yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
120 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 | D (+) glucose | 22 MILLIMOLAR | Role: Carbon source; Properties: Defined component, Simple component, Organic compound | |
| 5 |
KCl
Cl.K 74.551 g/mol
|
2.7 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.4 MILLIMOLAR |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.2 MILLIMOLAR |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
1.3 MILLIMOLAR |
CHEBI:37585
sodium dihydrogenphosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 10 | heat-inactivated fetal bovine serum | 5 PERCENT_W_V | Role: Nutrient source; Properties: Undefined component | |
| 11 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 12 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 13 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mitsuhashi_maramorosch_m_m_medium (CultureMech:008873)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.282014Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2287 |
| 2026-01-28T19:48:08.188677Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.941231Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008873 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.634531+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mitsuhashi-Maramorosch (M&M) medium" to "mitsuhashi_maramorosch_m_m_medium" for consistent matching |
| 2026-06-05T07:41:34.815069+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:46.527709+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 576407d215871db653a777fcb0db8855d0ecf4852ac605e4ac937f8fcfbc7871 |