Overview
- External ontology
- TOGO:M2287 — Mitsuhashi-Maramorosch (M&M) medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2287 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The false LB constituent expansion is withdrawn. Each source's actual hydrolysate and preparation scope is retained. Existing unrelated chemical annotations and history are preserved, not recertified; no taxa-media growth association is added.
Composition
flowchart LR
CultureMech_008873["mitsuhashi_maramorosch_m_m_medium"]
ing0_heat_inactivated_fetal_bovine_serum["heat-inactivated fetal bovine serum"]
CultureMech_008873 --> ing0_heat_inactivated_fetal_bovine_serum
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | heat-inactivated fetal bovine serum | Role: Nutrient source; Properties: Undefined component Source serum supplement is 5%, with no w/v, v/v or w/w basis. Withdraw the unsupported PERCENT_W_V value; preserve heat-inactivated fetal bovine serum and the unspecified percentage as text. |
Solutions
Mitsuhashi-Maramorosch basal medium
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1 L | Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component The source explicitly gives a 1 L water input, not 1 g/L or a measured final combined volume. |
| yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
| NaCl | 120 MILLIMOLAR | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
| D (+) glucose | 22 MILLIMOLAR | Role: Carbon source; Properties: Defined component, Simple component, Organic compound Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
| KCl | 2.7 MILLIMOLAR | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
| NaHCO3 | 1.4 MILLIMOLAR | Role: Buffer; Properties: Defined component, Inorganic compound, Simple component Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
| CaCl2 | 1 MILLIMOLAR | Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
| MgCl2 | 0.2 MILLIMOLAR | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
| NaH2PO4 | 1.3 MILLIMOLAR | Role: Buffer; Properties: Simple component, Defined component, Inorganic compound Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
| lactalbumin hydrolysate | 6.5 G_PER_L | Source-explicit basal-medium concentration before the separately described serum supplement. No final-medium dilution, molar/mass conversion or hydrate substitution is inferred. |
The source gives these basal concentrations before supplementation with 5% heat-inactivated fetal bovine serum. No basal-medium addition dose, final combined volume or dilution factor is supplied.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.282014Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2287 |
| 2026-01-28T19:48:08.188677Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.941231Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008873 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.634531+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mitsuhashi-Maramorosch (M&M) medium" to "mitsuhashi_maramorosch_m_m_medium" for consistent matching |
| 2026-06-05T07:41:34.815069+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-08T23:50:40.246918+00:00 | codex | RESTORED_SOURCE_SPECIFIC_HYDROLYSATE_FORMULATIONS_WITH_EVIDENCE | Restore source-specific hydrolysates, stock/basal/medium quantity boundaries, missing inputs and preparation; correct unsupported duplicate links. Preserve IDs and prior history. Unknown serum bases/doses and gas amounts remain unknown. |