Overview
- External ontology
- TOGO:M2328 — Middle Brook Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2328 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008914["middle_brook_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008914 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glycerol["Glycerol"]
CultureMech_008914 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_Bacto_Middlebrook_7H10_agar__Difco_262710_["Bacto Middlebrook 7H10 agar (Difco 262710)"]
CultureMech_008914 --> ing2_Bacto_Middlebrook_7H10_agar__Difco_262710_
ing3_Bacto_Middlebrook_OADC_enrichment["Bacto Middlebrook OADC enrichment"]
CultureMech_008914 --> ing3_Bacto_Middlebrook_OADC_enrichment
ing4_NaCl["NaCl"]
CultureMech_008914 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Dextrose["Dextrose"]
CultureMech_008914 --> ing5_Dextrose
CHEBI_17634["CHEBI:17634"]
ing5_Dextrose -.-> CHEBI_17634
ing6_Tryptone["Tryptone"]
CultureMech_008914 --> ing6_Tryptone
ing7_Yeast_extract["Yeast extract"]
CultureMech_008914 --> ing7_Yeast_extract
ing8_Sodium_chloride["Sodium chloride"]
CultureMech_008914 --> ing8_Sodium_chloride
ing8_Sodium_chloride -.-> CHEBI_26710
ing9_Oleic_acid["Oleic acid"]
CultureMech_008914 --> ing9_Oleic_acid
CHEBI_16196["CHEBI:16196"]
ing9_Oleic_acid -.-> CHEBI_16196
ing10_Catalase__beef_["Catalase (beef)"]
CultureMech_008914 --> ing10_Catalase__beef_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 900.0, 100.0] |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
5 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 3 | Bacto Middlebrook 7H10 agar (Difco 262710) | 19 G_PER_L | Properties: Defined component, Complex component | |
| 4 | Bacto Middlebrook OADC enrichment | 100 G_PER_L | ||
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.85 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
Dextrose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 8 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 9 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 10 |
Oleic acid
C18H34O2 282.468 g/mol
|
0.05 G_PER_L |
CHEBI:16196
oleic acid
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 11 | Catalase (beef) | 4 G_PER_L | Properties: Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- middle_brook_medium (CultureMech:008914)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.346022Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2328 |
| 2026-01-28T19:48:40.595325Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:40.149264Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.250115Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008914 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.962331+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middle Brook Medium" to "middle_brook_medium" for consistent matching |
| 2026-06-05T07:41:33.881391+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.171988+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:45.418708+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 19575a146e3d0053ea0e5872292ccbf35046a002fcf50fc846df4deda70fed51 |