Overview
- External ontology
- TOGO:M2328 — Middle Brook Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2328 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. False LB constituent expansion is withdrawn. Source-specific amounts and preparation boundaries supersede flattened concentrations. Existing chemical annotations and earlier history are preserved, not recertified; no organism-growth association is added. Source preparation context (main preparation): Sterilize for 15min by 121C cool to 50 - 55° C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly.
Composition
flowchart LR
CultureMech_008914["middle_brook_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008914 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glycerol["Glycerol"]
CultureMech_008914 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_Bacto_Middlebrook_7H10_agar__Difco_262710_["Bacto Middlebrook 7H10 agar (Difco 262710)"]
CultureMech_008914 --> ing2_Bacto_Middlebrook_7H10_agar__Difco_262710_
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
0.9 L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 900.0, 100.0] Source amount: 900 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 900 ml = 0.9 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it. |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
0.005 L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component Source amount: 5 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 5 ml = 0.005 L. |
| 3 | Bacto Middlebrook 7H10 agar (Difco 262710) | Properties: Defined component, Complex component Source amount: 19 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Solutions
Bacto Middlebrook OADC enrichment
Dose: 0.1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.1 L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 900.0, 100.0] Source amount: 100 ml; scope: Bacto Middlebrook OADC enrichment stock preparation. Volume-only input/dose, not a final-volume marker; 100 ml = 0.1 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it. |
| NaCl | Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component Source amount: 0.85 g; scope: Bacto Middlebrook OADC enrichment stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Dextrose | Role: Carbon source; Properties: Organic compound, Simple component, Defined component Source amount: 2 g; scope: Bacto Middlebrook OADC enrichment stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Albumin fraction V, bovine | Source amount: 5 g; scope: Bacto Middlebrook OADC enrichment stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Oleic acid | Role: Carbon source; Properties: Defined component, Organic compound, Simple component Source amount: 0.05 g; scope: Bacto Middlebrook OADC enrichment stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Catalase (beef) | Properties: Defined component, Simple component Source amount: 4 mg; scope: Bacto Middlebrook OADC enrichment stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Source amount: 100 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 100 ml = 0.1 L. Inline enrichment composition is separate from its dose and from finished-medium concentrations.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.346022Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2328 |
| 2026-01-28T19:48:40.595325Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:40.149264Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.250115Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008914 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.962331+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middle Brook Medium" to "middle_brook_medium" for consistent matching |
| 2026-06-05T07:41:33.881391+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.171988+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-09T00:43:54.768901+00:00 | codex | RESTORED_MIDDLEBROOK_INGREDIENT_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore albumin and separate basal inputs, commercial-base detail and ADC/OADC stock amounts/doses. Correct volume/mass confusion; retain unsupported mass/percent bases and source-version conflicts explicitly. Preserve IDs and all prior history. |