Overview
- External ontology
- TOGO:M2336 — Bacto Marine Broth (Difco 2216)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2336 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514.pdf
Composition
flowchart LR
CultureMech_008923["bacto_marine_broth_difco_2216"]
ing0_Distilled_water["Distilled water"]
CultureMech_008923 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008923 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_H3BO3["H3BO3"]
CultureMech_008923 --> ing2_H3BO3
CHEBI_33118["CHEBI:33118"]
ing2_H3BO3 -.-> CHEBI_33118
ing3_KCl["KCl"]
CultureMech_008923 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaHCO3["NaHCO3"]
CultureMech_008923 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Na2SO4["Na2SO4"]
CultureMech_008923 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6_CaCl2["CaCl2"]
CultureMech_008923 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_Na2HPO4["Na2HPO4"]
CultureMech_008923 --> ing7_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4 -.-> CHEBI_34683
ing8__NH4_NO3["(NH4)NO3"]
CultureMech_008923 --> ing8__NH4_NO3
CHEBI_63038["CHEBI:63038"]
ing8__NH4_NO3 -.-> CHEBI_63038
ing9_NaF["NaF"]
CultureMech_008923 --> ing9_NaF
CHEBI_28741["CHEBI:28741"]
ing9_NaF -.-> CHEBI_28741
ing10_KBr["KBr"]
CultureMech_008923 --> ing10_KBr
CHEBI_32030["CHEBI:32030"]
ing10_KBr -.-> CHEBI_32030
ing11_Fe_III__citrate["Fe(III) citrate"]
CultureMech_008923 --> ing11_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing11_Fe_III__citrate -.-> CHEBI_144421
ing12_SrCl2["SrCl2"]
CultureMech_008923 --> ing12_SrCl2
CHEBI_36383["CHEBI:36383"]
ing12_SrCl2 -.-> CHEBI_36383
ing13_Na_silicate["Na-silicate"]
CultureMech_008923 --> ing13_Na_silicate
CHEBI_60720["CHEBI:60720"]
ing13_Na_silicate -.-> CHEBI_60720
ing14_MgCl2__anhydrous_["MgCl2 (anhydrous)"]
CultureMech_008923 --> ing14_MgCl2__anhydrous_
ing15_Bacto_yeast_extract["Bacto yeast extract"]
CultureMech_008923 --> ing15_Bacto_yeast_extract
ing16_Bacto_peptone["Bacto peptone"]
CultureMech_008923 --> ing16_Bacto_peptone
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
19.45 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
H3BO3
H3BO3 61.833 g/mol
|
22 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.55 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.24 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1.8 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
8 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
(NH4)NO3
H4N.NO3 80.043 g/mol
|
1.6 G_PER_L |
CHEBI:63038
ammonium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaF
F.Na 41.988 g/mol
|
2.4 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
KBr
Br.K 119.002 g/mol
|
0.08 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.1 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 13 |
SrCl2
2Cl.Sr 158.526 g/mol
|
34 G_PER_L |
CHEBI:36383
strontium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na-silicate
2Na.O3Si 122.063 g/mol
|
4 G_PER_L |
CHEBI:60720
sodium silicate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 | MgCl2 (anhydrous) | 5.9 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 16 | Bacto yeast extract | 1 G_PER_L | Properties: Undefined component | |
| 17 | Bacto peptone | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- TOGO_M2336_Bacto_Marine_Broth_Difco_2216 (CultureMech:008923)
- TOGO_M2608_Bacto_Marine_Broth_Difco_2216 (CultureMech:009175)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.361788Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2336 |
| 2026-01-28T19:48:39.976218Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:01.309278Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008923 |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.025486+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Bacto Marine Broth (Difco 2216)" to "bacto_marine_broth_difco_2216" for consistent matching |
| 2026-06-05T07:40:36.305765+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.867753+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:11.272103+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: TOGO_M2336_Bacto_Marine_Broth_Difco_2216, TOGO_M2608_Bacto_Marine_Broth_Difco_2216 |
| 2026-08-06T03:05:11.272141+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 5181fe09f48a3e6bfc5d303630258a70fd4244ab4f3df74109a78dd609d49a94 |