bacto_marine_broth_difco_2216 (CultureMech:008923)

Overview

External ontology

TOGO:M2336 — Bacto Marine Broth (Difco 2216)

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2336 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514.pdf

Composition

flowchart LR
    CultureMech_008923["bacto_marine_broth_difco_2216"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008923 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_008923 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_H3BO3["H3BO3"]
    CultureMech_008923 --> ing2_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing2_H3BO3 -.-> CHEBI_33118
    ing3_KCl["KCl"]
    CultureMech_008923 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaHCO3["NaHCO3"]
    CultureMech_008923 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Na2SO4["Na2SO4"]
    CultureMech_008923 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_CaCl2["CaCl2"]
    CultureMech_008923 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_Na2HPO4["Na2HPO4"]
    CultureMech_008923 --> ing7_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing7_Na2HPO4 -.-> CHEBI_34683
    ing8__NH4_NO3["(NH4)NO3"]
    CultureMech_008923 --> ing8__NH4_NO3
    CHEBI_63038["CHEBI:63038"]
    ing8__NH4_NO3 -.-> CHEBI_63038
    ing9_NaF["NaF"]
    CultureMech_008923 --> ing9_NaF
    CHEBI_28741["CHEBI:28741"]
    ing9_NaF -.-> CHEBI_28741
    ing10_KBr["KBr"]
    CultureMech_008923 --> ing10_KBr
    CHEBI_32030["CHEBI:32030"]
    ing10_KBr -.-> CHEBI_32030
    ing11_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_008923 --> ing11_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing11_Fe_III__citrate -.-> CHEBI_144421
    ing12_SrCl2["SrCl2"]
    CultureMech_008923 --> ing12_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing12_SrCl2 -.-> CHEBI_36383
    ing13_Na_silicate["Na-silicate"]
    CultureMech_008923 --> ing13_Na_silicate
    CHEBI_60720["CHEBI:60720"]
    ing13_Na_silicate -.-> CHEBI_60720
    ing14_MgCl2__anhydrous_["MgCl2 (anhydrous)"]
    CultureMech_008923 --> ing14_MgCl2__anhydrous_
    ing15_Bacto_yeast_extract["Bacto yeast extract"]
    CultureMech_008923 --> ing15_Bacto_yeast_extract
    ing16_Bacto_peptone["Bacto peptone"]
    CultureMech_008923 --> ing16_Bacto_peptone

Ingredients (17)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1000 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2	NaCl Cl.Na 58.443 g/mol	19.45 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	H3BO3 H3BO3 61.833 g/mol	22 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	KCl Cl.K 74.551 g/mol	0.55 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5	NaHCO3 CHO3.Na 84.006 g/mol	0.16 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6	Na2SO4 2Na.O4S 142.043 g/mol	3.24 G_PER_L	CHEBI:32149 sodium sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7	CaCl2 Ca.2Cl 110.984 g/mol	1.8 G_PER_L	CHEBI:3312 calcium dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8	Na2HPO4 HO4P.2Na 141.958 g/mol	8 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9	(NH4)NO3 H4N.NO3 80.043 g/mol	1.6 G_PER_L	CHEBI:63038 ammonium nitrate	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
10	NaF F.Na 41.988 g/mol	2.4 G_PER_L	CHEBI:28741 sodium fluoride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11	KBr Br.K 119.002 g/mol	0.08 G_PER_L	CHEBI:32030 potassium bromide	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12	Fe(III) citrate C6H5FeO7 244.944 g/mol	0.1 G_PER_L	CHEBI:144421 iron(III) citrate	Role: Mineral source; Properties: Organic compound, Defined component, Simple component
13	SrCl2 2Cl.Sr 158.526 g/mol	34 G_PER_L	CHEBI:36383 strontium dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14	Na-silicate 2Na.O3Si 122.063 g/mol	4 G_PER_L	CHEBI:60720 sodium silicate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15	MgCl2 (anhydrous)	5.9 G_PER_L		Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16	Bacto yeast extract	1 G_PER_L		Properties: Undefined component
17	Bacto peptone	5 G_PER_L		Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.361788Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2336
2026-01-28T19:48:39.976218Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.309278Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008923
2026-03-17T06:30:50.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.025486+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Bacto Marine Broth (Difco 2216)" to "bacto_marine_broth_difco_2216" for consistent matching
2026-06-05T07:40:36.305765+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:48.867753+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.