Overview
- External ontology
- TOGO:M2366 — BSK-Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2366 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium403.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008950["bsk_medium"]
ing0_Aqua_bidest__filter_sterilized["Aqua bidest. filter-sterilized"]
CultureMech_008950 --> ing0_Aqua_bidest__filter_sterilized
ing1_Glucose______D__["Glucose (α-, D+)"]
CultureMech_008950 --> ing1_Glucose______D__
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_008950 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_Na_pyruvate["Na-pyruvate"]
CultureMech_008950 --> ing3_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Na_pyruvate -.-> CHEBI_50144
ing4_Na_citrate["Na-citrate"]
CultureMech_008950 --> ing4_Na_citrate
ing5_N_Acetylglucosamine["N-Acetylglucosamine"]
CultureMech_008950 --> ing5_N_Acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing5_N_Acetylglucosamine -.-> CHEBI_59640
ing6_Proteose_yeastolate__TC___DIFCO_5577_15_["Proteose yeastolate (TC) (DIFCO 5577-15)"]
CultureMech_008950 --> ing6_Proteose_yeastolate__TC___DIFCO_5577_15_
ing7_HEPES_buffer__acid___SIGMA_H3375_["HEPES buffer (acid) (SIGMA H3375)"]
CultureMech_008950 --> ing7_HEPES_buffer__acid___SIGMA_H3375_
ing8_L_Glutamine["L-Glutamine"]
CultureMech_008950 --> ing8_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing8_L_Glutamine -.-> CHEBI_18050
ing9_Proteose_tryptone__DIFCO_0123_01_["Proteose tryptone (DIFCO 0123-01)"]
CultureMech_008950 --> ing9_Proteose_tryptone__DIFCO_0123_01_
ing10_CMRL_1066_10_x_Glutamin__GIBCO_042_1545_["CMRL 1066/10 x Glutamin (GIBCO 042-1545)"]
CultureMech_008950 --> ing10_CMRL_1066_10_x_Glutamin__GIBCO_042_1545_
ing11_Proteose_peptone_No_2__DIFCO_0121_01_3_["Proteose peptone No 2 (DIFCO 0121-01-3)"]
CultureMech_008950 --> ing11_Proteose_peptone_No_2__DIFCO_0121_01_3_
ing12_NaOH["NaOH"]
CultureMech_008950 --> ing12_NaOH
CHEBI_32145["CHEBI:32145"]
ing12_NaOH -.-> CHEBI_32145
ing13_gelatine["gelatine"]
CultureMech_008950 --> ing13_gelatine
CHEBI_5291["CHEBI:5291"]
ing13_gelatine -.-> CHEBI_5291
ing14_bidest__Water["bidest. Water"]
CultureMech_008950 --> ing14_bidest__Water
ing15_Tryptone["Tryptone"]
CultureMech_008950 --> ing15_Tryptone
ing16_Yeast_extract["Yeast extract"]
CultureMech_008950 --> ing16_Yeast_extract
ing17_Sodium_chloride["Sodium chloride"]
CultureMech_008950 --> ing17_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing17_Sodium_chloride -.-> CHEBI_26710
ing18_Rabbit_serum__inactivated_GIBCO_037_06120M_["Rabbit serum, inactivated(GIBCO 037-06120M)"]
CultureMech_008950 --> ing18_Rabbit_serum__inactivated_GIBCO_037_06120M_
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Aqua bidest. filter-sterilized | 900 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component | |
| 2 | Glucose (α-, D+) | 3 G_PER_L | Role: Carbon source; Properties: Defined component, Simple component, Organic compound | |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.8 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 5 | Na-citrate | 0.7 G_PER_L | Role: Buffer; Properties: Simple component, Defined component, Organic compound | |
| 6 | N-Acetylglucosamine | 0.4 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 7 | Proteose yeastolate (TC) (DIFCO 5577-15) | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 8 | HEPES buffer (acid) (SIGMA H3375) | 6 G_PER_L | Role: Buffer; Properties: Defined component, Simple component, Organic compound | |
| 9 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
0.1 G_PER_L |
CHEBI:18050
L-glutamine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 10 | Proteose tryptone (DIFCO 0123-01) | 1 G_PER_L | Properties: Undefined component | |
| 11 | CMRL 1066/10 x Glutamin (GIBCO 042-1545) | 100 G_PER_L | Properties: Defined component, Complex component | |
| 12 | Proteose peptone No 2 (DIFCO 0121-01-3) | 5 G_PER_L | Properties: Complex component | |
| 13 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 14 | gelatine | 14 G_PER_L |
CHEBI:5291
gelatin
|
Role: Solidifying component; Properties: Undefined component |
| 15 | bidest. Water | 343.0 G_PER_L | Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 200.0, 143.0] | |
| 16 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 17 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 18 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 19 | Rabbit serum, inactivated(GIBCO 037-06120M) | 86 G_PER_L | Role: Nutrient source; Properties: Undefined component |
Solutions
Solution A:
Dose: 1000 G_PER_L
Solution B:
Dose: 200 G_PER_L
Solution C:
Dose: 143 G_PER_L
Solution D:
Dose: 86 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2366_BSK-Medium (CultureMech:008950)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.402537Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2366 |
| 2026-01-28T19:48:09.314134Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:15.069199+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:42.236957Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.513504Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008950 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.236893+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BSK-Medium" to "bsk_medium" for consistent matching |
| 2026-05-16T09:03:13.283456+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.517335+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.937537+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:11.630757+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 55a97aaf4d78c85ff9efa94832c930c7e30b464ce63b4e1d44690ed2da5b20ee |