Overview
- External ontology
- TOGO:M2366 — BSK-Medium
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: https://togomedium.org/medium/M2366 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium403.pdf
Ingredients (0)
No ingredients recorded.
Preparation
- Warm Solutions A, B, C, and D to 37 C.
- Combine warm Solutions A, B, C, and D. Solution B may instead be added after filtration.
- Filter-sterilize the mixed medium while warm.
Solutions
Solution A
Dose: 699.790 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Aqua bidest. filter-sterilized | 900 ML_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| Glucose (α-, D+) | 3 G_PER_L | Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| MgCl2 x 6 H2O | 0.3 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| Na-pyruvate | 0.8 G_PER_L | Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| Na-citrate | 0.7 G_PER_L | Role: Buffer; Properties: Simple component, Defined component, Organic compound |
| N-Acetylglucosamine | 0.4 G_PER_L | Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| Proteose yeastolate (TC) (DIFCO 5577-15) | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component |
| HEPES buffer (acid) (SIGMA H3375) | 6 G_PER_L | Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| L-Glutamine | 0.1 G_PER_L | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| Proteose tryptone (DIFCO 0123-01) | 1 G_PER_L | Properties: Undefined component |
| CMRL 1066/10 x Glutamin (GIBCO 042-1545) | 100 ML_PER_L | Properties: Defined component, Complex component |
| Proteose peptone No 2 (DIFCO 0121-01-3) | 5 G_PER_L | Properties: Complex component |
Source batch uses 1000 ml in 1429 ml total; normalized to ml/L.
Solution B
Dose: 139.958 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| gelatine | 70 G_PER_L | Role: Solidifying component; Properties: Undefined component |
| bidest. Water | 1000 ML_PER_L | Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
Source batch uses 200 ml in 1429 ml total; normalized to ml/L.
Solution C
Dose: 100.070 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| bidest. Water | 1000 ML_PER_L | Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component |
| bovine serum albumine, fract. V (important: Sigma No A9647) | 350 G_PER_L | Role: Nutrient source |
Source batch uses 143 ml in 1429 ml total; normalized to ml/L.
Solution D
Dose: 60.182 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Rabbit serum, inactivated(GIBCO 037-06120M) | 1000 ML_PER_L | Role: Nutrient source; Properties: Undefined component |
Source batch uses 86 ml in 1429 ml total; normalized to ml/L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.402537Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2366 |
| 2026-01-28T19:48:09.314134Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:15.069199+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:42.236957Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.513504Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008950 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.236893+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BSK-Medium" to "bsk_medium" for consistent matching |
| 2026-05-16T09:03:13.283456+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.517335+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.937537+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-25T00:00:00-07:00 | repair_togo_bsk_structure.py | RESTORED_TOGO_STOCK_SOLUTION_BOUNDARIES | Rebuilt TOGO M2366 from the checked-in TOGO payload and DSMZ Medium 403. Restored Solutions A-D, bovine serum albumin, source units and preparation instructions; removed three unrelated LB constituents. Normalized the 1429 ml source batch to ml/L. |