Overview
- External ontology
- TOGO:M2402 — Middlebrook 7H9 broth supplemented with oleic acid-albumin-dextrose-catalase (Difco)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2402 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008985["middlebrook_7h9_broth_supplemented_with_oleic_acid_albumin_dextrose..."]
ing0_Glycerol["Glycerol"]
CultureMech_008985 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_DI_Water["DI Water"]
CultureMech_008985 --> ing1_DI_Water
ing2_Middlebrook_7H9_broth["Middlebrook 7H9 broth"]
CultureMech_008985 --> ing2_Middlebrook_7H9_broth
ing3_Tryptone["Tryptone"]
CultureMech_008985 --> ing3_Tryptone
ing4_Yeast_extract["Yeast extract"]
CultureMech_008985 --> ing4_Yeast_extract
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_008985 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Monopotassium_Phosphate["Monopotassium Phosphate"]
CultureMech_008985 --> ing6_Monopotassium_Phosphate
CHEBI_63036["CHEBI:63036"]
ing6_Monopotassium_Phosphate -.-> CHEBI_63036
ing7_Biotin["Biotin"]
CultureMech_008985 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Ammonium_Sulfate["Ammonium Sulfate"]
CultureMech_008985 --> ing8_Ammonium_Sulfate
CHEBI_62946["CHEBI:62946"]
ing8_Ammonium_Sulfate -.-> CHEBI_62946
ing9_Calcium_Chloride["Calcium Chloride"]
CultureMech_008985 --> ing9_Calcium_Chloride
CHEBI_86158["CHEBI:86158"]
ing9_Calcium_Chloride -.-> CHEBI_86158
ing10_Disodium_Phosphate["Disodium Phosphate"]
CultureMech_008985 --> ing10_Disodium_Phosphate
CHEBI_34683["CHEBI:34683"]
ing10_Disodium_Phosphate -.-> CHEBI_34683
ing11_Magnesium_Sulfate["Magnesium Sulfate"]
CultureMech_008985 --> ing11_Magnesium_Sulfate
CHEBI_32599["CHEBI:32599"]
ing11_Magnesium_Sulfate -.-> CHEBI_32599
ing12_Copper_Sulfate["Copper Sulfate"]
CultureMech_008985 --> ing12_Copper_Sulfate
CHEBI_23414["CHEBI:23414"]
ing12_Copper_Sulfate -.-> CHEBI_23414
ing13_Sodium_Citrate["Sodium Citrate"]
CultureMech_008985 --> ing13_Sodium_Citrate
CHEBI_53258["CHEBI:53258"]
ing13_Sodium_Citrate -.-> CHEBI_53258
ing14_Ferric_Ammonium_Citrate["Ferric Ammonium Citrate"]
CultureMech_008985 --> ing14_Ferric_Ammonium_Citrate
CHEBI_31604["CHEBI:31604"]
ing14_Ferric_Ammonium_Citrate -.-> CHEBI_31604
ing15_L_Glutamic_Acid["L-Glutamic Acid"]
CultureMech_008985 --> ing15_L_Glutamic_Acid
CHEBI_16015["CHEBI:16015"]
ing15_L_Glutamic_Acid -.-> CHEBI_16015
ing16_Pyridoxine["Pyridoxine"]
CultureMech_008985 --> ing16_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing16_Pyridoxine -.-> CHEBI_16709
ing17_Zinc_Sulfate["Zinc Sulfate"]
CultureMech_008985 --> ing17_Zinc_Sulfate
CHEBI_35176["CHEBI:35176"]
ing17_Zinc_Sulfate -.-> CHEBI_35176
ing18_NaCl["NaCl"]
CultureMech_008985 --> ing18_NaCl
ing18_NaCl -.-> CHEBI_26710
ing19_Dextrose__BD_215530_["Dextrose (BD 215530)"]
CultureMech_008985 --> ing19_Dextrose__BD_215530_
ing20_Oleic_Acid__Sigma_Cat___O_1008_["Oleic Acid (Sigma Cat # O-1008)"]
CultureMech_008985 --> ing20_Oleic_Acid__Sigma_Cat___O_1008_
ing21_Catalase__beef___Sigma_C_9322_["Catalase (beef) (Sigma C-9322)"]
CultureMech_008985 --> ing21_Catalase__beef___Sigma_C_9322_
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
2 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 2 | DI Water | 1000.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 900.0, 100.0] | |
| 3 | Middlebrook 7H9 broth | 4.7 G_PER_L | ||
| 4 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 7 |
Monopotassium Phosphate
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 9 |
Ammonium Sulfate
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Calcium Chloride
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Disodium Phosphate
HO4P.2Na 141.958 g/mol
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Magnesium Sulfate
Mg.O4S 120.368 g/mol
|
0.05 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Copper Sulfate
Cu.O4S 159.609 g/mol
|
1 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Sodium Citrate
C6H5O7.3Na 258.069 g/mol
|
0.1 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 15 | Ferric Ammonium Citrate | 0.04 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 16 |
L-Glutamic Acid
C5H9NO4 147.13 g/mol
|
0.5 G_PER_L |
CHEBI:16015
L-glutamic acid
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 17 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
1 G_PER_L |
CHEBI:16709
pyridoxine
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Zinc Sulfate
O4S.Zn 161.453 g/mol
|
1 G_PER_L |
CHEBI:35176
zinc sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NaCl
Cl.Na 58.443 g/mol
|
0.85 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 | Dextrose (BD 215530) | 2 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 21 | Oleic Acid (Sigma Cat # O-1008) | 0.05 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 22 | Catalase (beef) (Sigma C-9322) | 0.004 G_PER_L | Properties: Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- middlebrook_7h9_broth_supplemented_with_oleic_acid_albumin_dextrose_catalase_difco (CultureMech:008985)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.464732Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2402 |
| 2026-01-28T19:48:28.127367Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:17.131415Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.816762Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008985 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.560512+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middlebrook 7H9 broth supplemented with oleic acid-albumin-dextrose-catalase (Difco)" to "middlebrook_7h9_broth_supplemented_with_oleic_acid_albumin_dextrose_catalase_difco" for consistent matching |
| 2026-06-05T07:41:33.977162+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.243616+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:45.512351+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 025aef6221dffee6e8b246cd33c29160942617caa8a7ba504711154c0388fe92 |
| 2026-08-15T20:09:30.438536+00:00 | dedupe_identical_ingredients.py | REMOVED_DUPLICATE_INGREDIENT_ROWS | Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract. |