Overview
- External ontology
- TOGO:M2402 — Middlebrook 7H9 broth supplemented with oleic acid-albumin-dextrose-catalase (Difco)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2402 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. False LB constituent expansion is withdrawn. Source-specific amounts and preparation boundaries supersede flattened concentrations. Existing chemical annotations and earlier history are preserved, not recertified; no organism-growth association is added. Source preparation context (main preparation): Add and mix all components except for OADC. Bring to a boil to dissolve agar and autoclave at 121ºC. Cool to 50-55ºC and aseptically OADC. Source conflict: this broth table has no agar row, although the prose mentions dissolving agar. No agar is added by inference.
Composition
flowchart LR
CultureMech_008985["middlebrook_7h9_broth_supplemented_with_oleic_acid_albumin_dextrose..."]
ing0_Glycerol["Glycerol"]
CultureMech_008985 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_DI_Water["DI Water"]
CultureMech_008985 --> ing1_DI_Water
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
0.002 L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 2 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 2 ml = 0.002 L. |
| 2 | DI Water | 0.9 L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 900.0, 100.0] Source amount: 900 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 900 ml = 0.9 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it. |
Solutions
Middlebrook 7H9 broth
| Ingredient | Concentration | Notes |
|---|---|---|
| Monopotassium Phosphate | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Biotin | Role: Vitamin; Properties: Organic compound, Defined component, Simple component Source amount: 0.5 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Ammonium Sulfate | Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.5 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Calcium Chloride | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.5 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Disodium Phosphate | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source amount: 2.5 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Magnesium Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.05 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Copper Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Sodium Citrate | Role: Buffer; Properties: Organic compound, Defined component, Simple component Source amount: 0.1 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Ferric Ammonium Citrate | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component Source amount: 0.04 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| L-Glutamic Acid | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component Source amount: 0.5 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Pyridoxine | Role: Vitamin; Properties: Organic compound, Defined component, Simple component Source amount: 1 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Zinc Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Source amount: 4.7 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. Inline commercial-base formula detail, not extra simultaneous ingredients. Its table masses have no verified volume/dilution basis.
Dubos Oleic Albumin Complex (OADC)
Dose: 0.1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.85 g; scope: Dubos Oleic Albumin Complex (OADC) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Dextrose (BD 215530) | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 2 g; scope: Dubos Oleic Albumin Complex (OADC) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Albumin Fraction V Bovine (Sigma A9647) | Source amount: 5 g; scope: Dubos Oleic Albumin Complex (OADC) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| DI Water | 0.1 L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 900.0, 100.0] Source amount: 100 ml; scope: Dubos Oleic Albumin Complex (OADC) stock preparation. Volume-only input/dose, not a final-volume marker; 100 ml = 0.1 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it. |
| Oleic Acid (Sigma Cat # O-1008) | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 0.05 g; scope: Dubos Oleic Albumin Complex (OADC) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Catalase (beef) (Sigma C-9322) | Properties: Defined component, Simple component Source amount: 0.004 g; scope: Dubos Oleic Albumin Complex (OADC) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Source amount: 100 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 100 ml = 0.1 L. Inline enrichment composition is separate from its dose and from finished-medium concentrations.
Source preparation context (Dubos Oleic Albumin Complex (OADC)): Filter sterilize.
Source preparation context (Dubos Oleic Albumin Complex (OADC)): OADC is available from BD (237510) or can be made following the formulation above.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.464732Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2402 |
| 2026-01-28T19:48:28.127367Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:17.131415Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.816762Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008985 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.560512+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middlebrook 7H9 broth supplemented with oleic acid-albumin-dextrose-catalase (Difco)" to "middlebrook_7h9_broth_supplemented_with_oleic_acid_albumin_dextrose_catalase_difco" for consistent matching |
| 2026-06-05T07:41:33.977162+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.243616+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-15T20:08:38.740487+00:00 | dedupe_identical_ingredients.py | REMOVED_DUPLICATE_INGREDIENT_ROWS | Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract. |
| 2026-10-09T00:43:54.768901+00:00 | codex | RESTORED_MIDDLEBROOK_INGREDIENT_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore albumin and separate basal inputs, commercial-base detail and ADC/OADC stock amounts/doses. Correct volume/mass confusion; retain unsupported mass/percent bases and source-version conflicts explicitly. Preserve IDs and all prior history. |