Overview
- External ontology
- TOGO:M2408 — Modified Leptospira Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2408 Original URL: https://www.atcc.org/~/media/D1844FEFC4614D7FAFD9CD3C4FE12C43.ashx
Composition
flowchart LR
CultureMech_008991["modified_leptospira_medium"]
ing0_NaCl["NaCl"]
CultureMech_008991 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_DI_Water["DI Water"]
CultureMech_008991 --> ing1_DI_Water
ing2_Agar["Agar"]
CultureMech_008991 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Beef_Extract["Beef Extract"]
CultureMech_008991 --> ing3_Beef_Extract
ing4_Peptone["Peptone"]
CultureMech_008991 --> ing4_Peptone
ing5_Supplement_A["Supplement A"]
CultureMech_008991 --> ing5_Supplement_A
ing6_Sterile_Rabbit_Serum["Sterile Rabbit Serum"]
CultureMech_008991 --> ing6_Sterile_Rabbit_Serum
ing7_Distilled_water["Distilled water"]
CultureMech_008991 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
ing8_Hemin["Hemin"]
CultureMech_008991 --> ing8_Hemin
CHEBI_50385["CHEBI:50385"]
ing8_Hemin -.-> CHEBI_50385
ing9_NaOH["NaOH"]
CultureMech_008991 --> ing9_NaOH
CHEBI_32145["CHEBI:32145"]
ing9_NaOH -.-> CHEBI_32145
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 2 | DI Water | 900 G_PER_L | Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component | |
| 3 | Agar | 1.5 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 4 | Beef Extract | 0.2 G_PER_L | Properties: Undefined component | |
| 5 | Peptone | 0.3 G_PER_L | ||
| 6 | Supplement A | 100 G_PER_L | ||
| 7 | Sterile Rabbit Serum | 100 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 8 |
Distilled water
H2O 18.015 g/mol
|
100 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
0.05 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Organic compound, Simple component, Defined component |
| 10 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
0.05% Hemin solution
Dose: 2.5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_leptospira_medium (CultureMech:008991)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.477283Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2408 |
| 2026-01-28T19:48:27.710655Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:45.832262+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:01.868070Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008991 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.613093+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Leptospira Medium" to "modified_leptospira_medium" for consistent matching |
| 2026-05-16T09:04:25.480546+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:36.794887+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.487431+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f41848b886cb4a43a2e602f106151b66027b73c5d23b26a0d52d37896fd092dc |