Overview
- External ontology
- TOGO:M2410 — BSK II Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2410 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008994["bsk_ii_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008994 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_sodium_bicarbonate["sodium bicarbonate"]
CultureMech_008994 --> ing1_sodium_bicarbonate
CHEBI_32139["CHEBI:32139"]
ing1_sodium_bicarbonate -.-> CHEBI_32139
ing2_sodium_pyruvate["sodium pyruvate"]
CultureMech_008994 --> ing2_sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_sodium_pyruvate -.-> CHEBI_50144
ing3_sodium_citrate["sodium citrate"]
CultureMech_008994 --> ing3_sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing3_sodium_citrate -.-> CHEBI_53258
ing4_Tryptone["Tryptone"]
CultureMech_008994 --> ing4_Tryptone
ing5_Yeast_extract["Yeast extract"]
CultureMech_008994 --> ing5_Yeast_extract
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_008994 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Yeastolate__Difco_["Yeastolate (Difco)"]
CultureMech_008994 --> ing7_Yeastolate__Difco_
ing8_N_acetylglucosamine__Sigma_["N-acetylglucosamine (Sigma)"]
CultureMech_008994 --> ing8_N_acetylglucosamine__Sigma_
ing9_N_2_hydroxyethylpiperazine_N_2_ethanesulfonic_acid__HEPES__Sigma_Chemical_Co___St__Louis__MO_["N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES, Sigma Ch..."]
CultureMech_008994 --> ing9_N_2_hydroxyethylpiperazine_N_2_ethanesulfonic_acid__HEPES__Sigma_Chemical_Co___St__Louis__MO_
ing10_glucose["glucose"]
CultureMech_008994 --> ing10_glucose
CHEBI_17234["CHEBI:17234"]
ing10_glucose -.-> CHEBI_17234
ing11_10X_concentrate_of_CMRL_1066_without_glutamine__Gibco_Laboratories__GrandIsland__NY__No_330_1540_["10X concentrate of CMRL 1066 without glutamine (Gibco Laboratories,..."]
CultureMech_008994 --> ing11_10X_concentrate_of_CMRL_1066_without_glutamine__Gibco_Laboratories__GrandIsland__NY__No_330_1540_
ing12_Neopeptone__Difco_Laboratories__Detroit__Ml_["Neopeptone (Difco Laboratories, Detroit, Ml)"]
CultureMech_008994 --> ing12_Neopeptone__Difco_Laboratories__Detroit__Ml_
ing13_NaOH["NaOH"]
CultureMech_008994 --> ing13_NaOH
CHEBI_32145["CHEBI:32145"]
ing13_NaOH -.-> CHEBI_32145
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
sodium bicarbonate
CHO3.Na 84.006 g/mol
|
2.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.8 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 4 |
sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.7 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 5 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 6 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 8 | Yeastolate (Difco) | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 9 | N-acetylglucosamine (Sigma) | 0.4 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 10 | N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES, Sigma Chemical Co., St. Louis, MO) | 6 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 11 |
glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 12 | 10X concentrate of CMRL 1066 without glutamine (Gibco Laboratories, GrandIsland, NY, No.330-1540) | 100 G_PER_L | Properties: Defined component, Complex component | |
| 13 | Neopeptone (Difco Laboratories, Detroit, Ml) | 5 G_PER_L | Properties: Undefined component | |
| 14 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- bsk_ii_medium (CultureMech:008994)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.479476Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2410 |
| 2026-01-28T19:48:30.918964Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-13T06:20:01.880605Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008994 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.626924+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BSK II Medium" to "bsk_ii_medium" for consistent matching |
| 2026-05-16T09:03:26.276749+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:51.652345+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:26.039391+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3729dbe6af23b738a93670f79ccfe3eb398cf8a4657e226798dce1015f3be422 |