bsk_ii_medium

Overview

External ontology

TOGO:M2410 — BSK II Medium

Applications

• Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2410 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The false LB constituent expansion is withdrawn in favor of this source's bovine serum albumin input. Existing unrelated chemical annotations are preserved, not recertified. This repair does not assert equivalence with other BSK formulations or add organism-growth evidence. 9. Incubate at 34-37°C *Hamilton, Montana, is at an altitude of 1,200m. At lower elevations the containiers may need to be filled to a higher level.

Composition

flowchart LR
    CultureMech_008994["bsk_ii_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008994 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_sodium_bicarbonate["sodium bicarbonate"]
    CultureMech_008994 --> ing1_sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing1_sodium_bicarbonate -.-> CHEBI_32139
    ing2_sodium_pyruvate["sodium pyruvate"]
    CultureMech_008994 --> ing2_sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing2_sodium_pyruvate -.-> CHEBI_50144
    ing3_sodium_citrate["sodium citrate"]
    CultureMech_008994 --> ing3_sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_sodium_citrate -.-> CHEBI_53258
    ing4_bovine_serum_albumin__FractionV__Miles_Laboratories__Elkhart__IN__No_81_003_["bovine serum albumin, FractionV (Miles Laboratories, Elkhart, IN, N..."]
    CultureMech_008994 --> ing4_bovine_serum_albumin__FractionV__Miles_Laboratories__Elkhart__IN__No_81_003_
    ing5_Yeastolate__Difco_["Yeastolate (Difco)"]
    CultureMech_008994 --> ing5_Yeastolate__Difco_
    ing6_N_acetylglucosamine__Sigma_["N-acetylglucosamine (Sigma)"]
    CultureMech_008994 --> ing6_N_acetylglucosamine__Sigma_
    ing7_N_2_hydroxyethylpiperazine_N_2_ethanesulfonic_acid__HEPES__Sigma_Chemical_Co___St__Louis__MO_["N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES, Sigma Ch..."]
    CultureMech_008994 --> ing7_N_2_hydroxyethylpiperazine_N_2_ethanesulfonic_acid__HEPES__Sigma_Chemical_Co___St__Louis__MO_
    ing8_glucose["glucose"]
    CultureMech_008994 --> ing8_glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_glucose -.-> CHEBI_17234
    ing9_Neopeptone__Difco_Laboratories__Detroit__Ml_["Neopeptone (Difco Laboratories, Detroit, Ml)"]
    CultureMech_008994 --> ing9_Neopeptone__Difco_Laboratories__Detroit__Ml_

Ingredients (10)

Preparation

1. 1. After detergent cleaning, all glassware is rinsed thoroughly with glass-distilled water and then autoclaved.
2. 2. To 900ml of glass-distilled water are added 100ml of 10X concentrate of CMRL 1066 without glutamine (Gibco Laboratories, GrandIsland, NY, No.330-1540).
3. Add the source-listed ingredients to the diluted CMRL 1066. The provider requests an addition order, but its item array is not accepted as that order; exact addition order remains unresolved here.
4. 4. Adjust pH of medium at 20-25°C to 7.6 with 1 N NaOH.
5. 5. Add 200ml of 7 percent gelatin (Difco) which had been dissolved in boilinig water.
6. 6. Sterilize by filtration with air pressure (0.2 μm nitrocellulose, Millipore Corp., Bedford, MA). Store medium at 4°C.
7. 7. Before use, add unheated rabbit serum(\"trace hemolyzed\"; Pel-Freez Biologicals, Inc., Rogers, AR) to a final concentration of 6 percent.
8. 8. Dispense to glass or polystyrene tubes or bottles. Fill containers to 50 to 90 percent capacity and captightly.*

Solutions

Target organisms

No target organisms are recorded.

Provenance