lb_broth_miller

CultureMech:009027 bacterial COMPLEX LIQUID
Original name: LB Broth, Miller

Overview

External ontology
TOGO:M2448 — LB Broth, Miller
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'lb_agar_broth_miller', 'source': 'TOGO', 'source_id': 'TOGO:M2199', 'original_category': 'bacterial'}
  • {'name': 'lb_agar_miller', 'source': 'TOGO', 'source_id': 'TOGO:M2446', 'original_category': 'bacterial'}
Notes
Source: https://togomedium.org/medium/M2448 Original URL: https://www.atcc.org/~/media/6F57EBDFF28049EE85F7C697DAFB2592.ashx Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_009027["lb_broth_miller"]
    ing0_DI_Water["DI Water"]
    CultureMech_009027 --> ing0_DI_Water
    ing1_Tryptone["Tryptone"]
    CultureMech_009027 --> ing1_Tryptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009027 --> ing2_Yeast_extract
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_009027 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Yeast_Extract["Yeast Extract"]
    CultureMech_009027 --> ing4_Yeast_Extract
    ing5_Sodium_Chloride["Sodium Chloride"]
    CultureMech_009027 --> ing5_Sodium_Chloride
    ing5_Sodium_Chloride -.-> CHEBI_26710
    ing6_Tryptone["Tryptone"]
    CultureMech_009027 --> ing6_Tryptone

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 DI Water 1000 G_PER_L Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
10.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
3 Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
5.0 G_PER_L FOODON:03315426
yeast extract
 Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
4 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
10.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
5 Yeast Extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
6 Sodium Chloride
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
7 Tryptone 10 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:56.514249Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2448
2026-03-13T06:20:02.031426Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009027
2026-03-17T19:46:20.790180+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB Broth, Miller" to "lb_broth_miller" for consistent matching
2026-08-06T03:05:10.685910+00:00 recipe-merger Merged 3 duplicate recipes into canonical record Sources: TOGO_M2199_LB_Agar_Broth_Miller, lb_agar_miller, lb_broth_miller
2026-08-06T03:05:10.685947+00:00 merge_recipes.py MERGED_RECIPES merged 3 source recipe(s) on fingerprint 458b8df63152ec78dc712ec4dab5e7f947d80209d5ac468f990874d0aa380b79