Overview
- External ontology
- TOGO:M2448 — LB Broth, Miller
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2448 Original URL: https://www.atcc.org/~/media/6F57EBDFF28049EE85F7C697DAFB2592.ashx Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009027["lb_broth_miller"]
ing0_DI_Water["DI Water"]
CultureMech_009027 --> ing0_DI_Water
ing1_Tryptone["Tryptone"]
CultureMech_009027 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_009027 --> ing2_Yeast_extract
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_009027 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | DI Water | 1000 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component | |
| 2 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 3 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.514249Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2448 |
| 2026-03-13T06:20:02.031426Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009027 |
| 2026-03-17T19:46:20.790180+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB Broth, Miller" to "lb_broth_miller" for consistent matching |