Overview
- External ontology
- TOGO:M244 — Sulfophobococcus Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M244 Original source: JCM - JCM_M252 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=252 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009029["sulfophobococcus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009029 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009029 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009029 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_Resazurin["Resazurin"]
CultureMech_009029 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009029 --> ing4_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_9H2O -.-> CHEBI_76209
ing5_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009029 --> ing5_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_7H2O -.-> CHEBI_75836
ing6_KCl["KCl"]
CultureMech_009029 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_ZnCl2["ZnCl2"]
CultureMech_009029 --> ing7_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing7_ZnCl2 -.-> CHEBI_49976
ing8_Na2CO3["Na2CO3"]
CultureMech_009029 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_009029 --> ing9_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing9_MnSO4_xH2O -.-> CHEBI_86360
ing10_Tryptone["Tryptone"]
CultureMech_009029 --> ing10_Tryptone
ing11_Yeast_extract["Yeast extract"]
CultureMech_009029 --> ing11_Yeast_extract
ing12_Sodium_chloride["Sodium chloride"]
CultureMech_009029 --> ing12_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing12_Sodium_chloride -.-> CHEBI_26710
ing13_Glycine["Glycine"]
CultureMech_009029 --> ing13_Glycine
CHEBI_15428["CHEBI:15428"]
ing13_Glycine -.-> CHEBI_15428
ing14_Na2B4O7_10H2O["Na2B4O7・10H2O"]
CultureMech_009029 --> ing14_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing14_Na2B4O7_10H2O -.-> CHEBI_131366
ing15_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009029 --> ing15_Yeast_extract__BD_Difco_
ing16_EDTA_2Na["EDTA・2Na"]
CultureMech_009029 --> ing16_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing16_EDTA_2Na -.-> CHEBI_64734
ing17_N2["N2"]
CultureMech_009029 --> ing17_N2
CHEBI_17997["CHEBI:17997"]
ing17_N2 -.-> CHEBI_17997
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
31 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
66 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component |
| 5 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
50 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
31 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
2.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
230 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 10 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
2.3 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 11 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 12 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 13 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 14 |
Glycine
C2H5NO2 75.067 g/mol
|
1.5 G_PER_L |
CHEBI:15428
glycine
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 15 |
Na2B4O7・10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
1.8 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 16 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 17 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
32 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Simple component, Organic compound |
| 18 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M244_Sulfophobococcus_Medium (CultureMech:009029)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.692027Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M244 |
| 2026-01-28T19:48:36.151720Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:02.039959Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009029 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.798779+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sulfophobococcus Medium" to "sulfophobococcus_medium" for consistent matching |
| 2026-05-16T09:03:13.531835+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.802714+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:26.639719+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.025905+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:26.485588+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7f098ba54b16c7c52b4f54bc52c8366aafc8c0ad3bfbd7ae875484c727600e40 |